Hi everybody. I want to use an Agilent Seahorse XFe24 analyzer for my metabolic assays. My cells will be embedded in hydrogels to be able to design a specific environment for them. However, the probes (sensors) of the machine can be lowered up to 200um from the bottom of the plate. The height (200um) is very small for my hydrogels. When I checked the literature, I found that many articles use microbeads but their diameter was also more than 200um. I am not sure how they were able to take accurate measurement from the analyzer even though the height of their gels was more than it should. Is there any idea or anyone that had the same problem? Thanks for the advice.
Riaz A. Khan Sorry I couldn't understand what you meant by the possible limit.
Dear Nuriye Solcan
The best option will be to contact the technical support from Agilent. They will be able to answer you more accurately regarding the height range that is required for the sensors to work accurately. It might be an option to ask if they have specific plate set up that can be used for your hydrogels.
In case you haven't read it then I can suggest to read this protocol where the authors use organoids in the Xfe24 system- https://star-protocols.cell.com/protocols/521
You might get some ideas from here.
Good luck !
Shambhabi Chatterjee Thank you very much. I will definitely read this protocol. It looks promising. On the other hand, I had already contacted technical support but it was a dead end. I appreciate your help. Have a nice day.
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