Good evening
This is my second time experimenting with the comet assay. Specifically, we are running PBMC samples and using silver stain for visualization. Could this images observed under the light microscope (10x and 40x objective) be comets that can be evaluated?
Thank you in advance!
Hello Panagiota Resta
Yes, they are comets. In comet assay, under the microscope, you will observe structures resembling comet with a distinct head and tail. The head represents the intact DNA, which remains within the nucleus during the assay, while the tail consists of short fragments of DNA formed by the migration of DNA fragments that have been damaged or broken during the electrophoresis process. The length and intensity of the tail are indicative of the extent of DNA damage that have migrated during the electrophoresis process.
Best,
Panagiota Resta looks like a comet, You can run some positive and negative controls to evaluate your results.
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