I have a 11kb fragment and I am trying to do IVT but every time I perform IVT a smear like band observe also it doesn't match the size any solution?

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The authors are asked to provide and elaborate the explanations and comparison with the existing theoretical and/or experimental works related ?

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Aweza Sleman Qadir

I have the same problem, but with DNA? Can anyone know?

B R Siddharth

Aweza Sleman Qadir, please tell us more about the source of your DNA, whether it is a plasmid or a PCR product. Then, we can consider a possible solution. Can you share some images of the same? It will be helpful.