Hello everyone, I am trying to perform homologous recombination of the shuttle vector and pAd vector in BJ5183 cells. But it has been largely unsuccessful to date, and it is also difficult to distinguish the recombination as we always get PCR positive using primers for the sequence from parents (Shuttle and parent pAd vector) and the recombination product. If someone could share their experience with troubleshooting, it would be highly helpful.