Can we use DAD detector for Sucrose analysis?

25 March 2025 0 8K Report

Sucrose Analysis using DAD detector and what type of column we can use C18 or amide column?

Badges
Science method

More Abin Samson's questions See All

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How to determine anode and cathode materials for supercapacitor applications?

I observed that activated carbon is employed as anode materials and manganese oxide as a cathode material for supercapacitor application using two-electrode system in KOH electrolyte. Please...

26 April 2024 7,962 1 View

Why some materials store energy through cation while others through anions in two electrode system?

It is observed that MnO2 stores charge by means of potassium (+) ion while cobalt oxide store energy by hydroxide (-) ion while both materials are employed as cathodes in separate systems.

26 April 2024 8,079 1 View

How can i deal with flourescense inhomogeneity in MST protein-protein interaction?

I have tried several of these protein-protein interaction with different concentrations, but i don't get that average capillary scan. It always like the image uploaded. Please does anyone have a...

23 November 2023 1,078 0 View

Is there a structural explanation to why a same his-tag proteins may have different affinity to MST red-tris dye?

Both proteins are in PET28a(+) but one has high affinity (like 10 times higher) than the other his-tag protein. I am confused as to why it is so. Please does some have same experience?

16 November 2023 2,167 0 View

Which articles discuss foreign language policy implementation?

Please provide me with current, up to date peer-reviewed articles that discuss the image and reputation of the school when it offers foreign languages as a subject.

15 November 2023 7,509 0 View

What is the optimal design for loop antenna made of copper stiff wire to transmit at 81.39–90.00 kHz?

The research aims to devise an optimal copper loop antenna design specifically for testing magnetic field intensity within the frequency range of 81.39–90.00 kHz. The focus will be on selecting...

01 September 2023 7,392 1 View

Why the Warburb region in Nyquist plot is observed at the low frequency region?

The Nyquist plot obtained from the EIS spectroscopy shows the the mass transfer controlled region in the low frequency regime, which is observed as a non-vertical line in the plot. Please explain...

09 August 2023 2,610 1 View

How can I edit my publication dat in my publication ?

I uploaded my research articles as I publish but publication date was by mistakenly added wrong Now How to correct that please guide me

10 June 2023 9,186 1 View

Could I get articles on research on implementation of policy on ICT education and training in Kenya ?

These articles should be about the sequential steps in the implementation process, such as policy dissemination, capacity building, curriculum development, teacher training, infrastructure...

10 June 2023 9,088 2 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

How to clean the CAD detector?

The module of the CAD detector is corona Veo. It has been idle for a long time. And it was not well maintained before idle. I have replaced the gas filters and flushed the system with a 50/50...

01 August 2024 7,805 0 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,293 4 View