How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Why does the DNA's elution decrease after centrifuging with spin column?

02 January 2021 987 4 View

A three level hierarchical data structure in multilevel modelling, for multiple response question?

Im refering to Andy Field's example in his book Discovering statistics using SPSS, 3rd edition, page 728, where he gives an example of a data structure with level 1 variable is a memory recall...

26 December 2020 9,717 6 View

What is the reasons of increased th molecular weight of some chemicals in plant when the concentration of a pollutant that effectin on plant?

please need paper explain this case

23 December 2020 2,657 2 View

How microhardness is dependent on surface roughness?

Does polishing or finishing affect the microhardness of alloys and metal, if yes how are these characteristics are related empirically?

21 December 2020 1,198 3 View

RBC's contamination during DNA extraction ?

Hi there! currently i am working with spin column kit for DNA extraction I've put almost 600 uL of buffy coat. after a lot of washing, the RBC's remained stucking in filter? How could i get rid...

25 October 2020 8,712 4 View

How to measure internal validation of multiple regression using a split-sample method with dichotomous variables?

I would like to validate a regression model using a 50-50 (or 80-20) split method. In my initial multiple regression I have entered all predictor variables using 'enter' technique. My sample size...

20 October 2020 2,849 5 View

How to aggregate expert opinion for Fuzzy AHP methodology?

Any recommended method or approach? Appreciate the support.

11 October 2020 5,290 6 View

What are the psychological effects on children during the COVID 19 pandemic?

I am working on the Psychological impact of COVID 19 on children of health care workers. i need references and guidelines for research on this topic.

20 July 2020 2,791 19 View

I need a protocol for Cas9 expression in E coli?

Hello Dear researchers, I am trying to express Cas9 vector in E coli but finding difficulties. After IPTG induction I am unable to get any protein(Cas9) via His-tag chromatography. Also, there is...

17 June 2020 1,170 2 View

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02 March 2021 3,060 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View