Gel Electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and... | Contact experts in Gel Electrophoresis to get answers

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Questions related to Gel Electrophoresis

Are they any methods of purifying sub 100 base pair ssDNA sequences directly from gel electrophoresis?

Im working with DNA origami and I curious if it is possible to recover the staple strands from an agarose seperation of DNA structures and their components

06 September 2019 5,263 3 View

I run real time PCR and I find one peak but two bands in gel electrophoresis. what is the problem?

I run real time PCR and I find one peak(that is specific for my product) but when I run it in the gel, I saw two bands in gel electrophoresis. The expected size for my amplicon is 150bp, that is...

15 July 2019 9,642 5 View

How do I interpret a triallelic pattern in a diploid organism's microsatellite?

Hello, I'm doing PCR and gel electrophoresis on tetranucleotide microsatellite repeat of a species of diploid salamander. The gel results yield three bands in two sample (attached; red arrows),...

27 June 2019 9,129 2 View

In free-flow electrophoresis tray is it necessary that the electrodes be in touch with liquids (buffers) or can they be designed behind the chamber?

I am going to fabricate a free flow electrophoresis (FFE) chamber. The chamber will contains the sample plus buffers (electrolytes). In capillary electrophoresis, IEF or gel-based electrophoresis...

23 June 2019 9,436 3 View

Why the distance between bromophenol blue bands and xylene cyanol FF bands are close?

I am doing the gel electrophoresis to check my DNA samples, and I found that after adding loading buffer and start running, the distance between bromophenol blue bands and xylene cyanol FF bands...

05 June 2019 2,394 3 View

Why do no bands appear during glycoprotein staining?

Dear all, I'm trying to stain sugar groups attached to my protein with Glycoprotein staining (Thermo Scientific Pierce Glycoprotein Staining Kit #24562) after gel electrophoresis. I've added 4...

08 April 2019 3,374 2 View

Could anybody say what is the general porosity of PDMS , i mean pore size ? can we use PDMS for DNA run??

Could anybody say what is the general porosity of PDMS , i mean pore size ? can we use PDMS for DNA run?? one more thing what is the pore size of Whatmann filter paper??

08 April 2019 1,841 2 View

Can Laemmli buffer buffer be re-used for future samples?

Good day, I prepare Laemmli buffer 2X from Bio-Rad by adding 950uL of Laemmli buffer + 50uL of B-mercaptoethanol for a final volume of 1mL for SDS-PAGE. Since a lot of Laemmli buffer still...

06 April 2019 3,697 4 View

Question regarding PCR & bands on agarose gel?

I'm trying to detect hblA gene by using the PCR method as described by the journal"Enterotoxigenic Profiles of Food-Poisoning and Food-Borne Bacillus cereus Strains". When I run gel...

27 March 2019 5,558 11 View

How the repeat number is assessed from fragment size (in basepairs ) in exon 3 VNTR of Dopamine receptor D4 gene?

I am currently working on DRD4 exon 3 VNTR to analyse association with methamphetamine abuse.Actually I have two questions- 1) I am following several papers.According to the paper the fragment...

27 March 2019 5,721 3 View

Actually how many the limitations of TAE or TBE to use (re-use) in gel electrophoresis?

Normally, I have fresh prepared TAE/TBE buffer. But sometimes I see one of my colleagues who re-use it max. 3 times, Is it corret or not? Does it have any effect to the result? Thanks

20 March 2019 3,004 3 View

Does Voltage affect sample migration speed linearly in Gel Electrophoresis?

I am just curious whether there is a linear correlation between voltage and time (sample migration speed) in gel electrophoresis, and I might run my own little test. For example, would a sample...

12 January 2019 820 3 View

What does an extra, unclear band coupled with a clear band indicates in gel electrophoresis?

Hello, I'm using PCR to assess the differences between the banding patterns of a microsatellite in different salamander species. In my resulting gel, there's extra unclear bands underneath the...

22 December 2018 8,793 6 View

How to distinguish between 2 alleles with similar bp sizes in gel electrophoresis?

Hello, What technique or modification to the gel electrophoresis procedure do I need in order to increase the difference in distance travelled by two alleles with very similar sizes? In my gel...

22 December 2018 2,339 2 View

How can I determine if my protein is a dimer?

I work with a protein which shows cooperativity on kinetic assays, and others of its class (although with low similarity) are dimers. However, it's not described in literature for this one that I...

20 December 2018 9,485 38 View

How do I excise DNA band for sequencing?

Hi, May I know how am I going to prepare a gel-electrophoresis with 25uL in each well so that I can send for sequencing after excision?

29 November 2018 3,133 3 View

In SDS-PAGE , how possible to use freez-dry samples for loading in gel electrophoresis?

I freeze fungal mycelia of mushroom. In SDS-PAGE of total protein ,If I freeze mycelium I can used this sample? How should I prepare mycelium for electrophoresis? How should I make it for...

02 October 2018 8,102 5 View

Why my nanoparticle have positive charges but could not bind siRNA?

i made one nanorod with AuNR@CuS composition. I use layer-by-layer method to coat SDS and chitosan oligosaccharide sequentially on the surface of nanoparticles. After doing the DLS measurement, i...

09 August 2018 8,210 5 View

Now i'am asking if i can characterize my isolated cells using gel electrophoresis after doing conventional PCR for genes expressed by theses cells?

after isolation of my cells can i make characterization for these cells using gel electrophoresis after doing conventional PCR for certain genes known from literature that they are overexpressed...

30 July 2018 311 2 View

Can anybody suggest me commercially available albumin out kits for sample preparation for 2D GE?

I’m using human serum samples for proteomic analysis. Can anyone suggest me if using Agilent Multiple affinity albumin removal columns are suitable enough in case I have to proceed with 2D gel...

06 June 2018 2,192 2 View

DNA Ladder and Bands not separating enough during gel electrophoresis: distinguish 2.5kb to 4.8kb bands?

I have been struggling to resolve a 4.8kb for a sub-cloning experiment from a 2.2kb band (other smaller fragments haven't been an issue). When increasing percentage of low melting agarose gel...

02 May 2018 3,927 14 View

Lack of consistency between RNAseq and RT-qPCR results?

Hi all, I am trying to verify the fold change of 18 genes (early stationary phase E. coli, control vs. treated, reference used is 16s rRNA) observed in RNAseq results by RT-qPCR, but was unable...

16 April 2018 7,971 3 View

Has anyone had problem with single cell whole genome amplification?

Hello, I am experiencing problem with single cell whole genome amplification from 10-12 cells only 3 or 4 amplifies and I have a smear on gel electrophoresis, Can anyone help me? Best Regards

13 February 2018 3,333 5 View

What technique of gel electrophoresis should be used to separate molecules weighing > 1000 kda?

I need a technique to protein evaluation with molecular weight over 1000 kda.

07 February 2018 7,761 3 View

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