Gel Electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and... | Contact experts in Gel Electrophoresis to get answers

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Questions related to Gel Electrophoresis

Why DNA quantification using micro spectrometer like nanodrop and gel electrophoresis shows difference?

DNA quantification using micro spectrometer like nanodrop and gel electrophoresis shows difference in their results. why its higher with spectrometer? Can anyone please share their experience and...

06 December 2016 1,823 6 View

Why is PFGE so important for DNase-seq studies? And is there a way to circumvent it's use?

I'm currently performing a DNase-seq study on some plant material but do not have access to PFGE machinery. As I understand it the PFGE is required to separate the HMW DNA, so that one can be sure...

23 November 2016 2,826 3 View

How to design experiment for 2D-DIGE with only 2 type of samples?

I want to design an experiment for 2D-DIGE and I have two types of samples, one: control tissue from healthy individuals and other from patients. besides comparing proteom of patients to control...

03 November 2016 3,599 2 View

How much further would a supercoiled plasmid migrate?

I'm validating if my clone was successful. I first made a colony PCR and the correct band appears. I was then told to validate further using restriction digestion. I added 15 uL of aprox 16 ng/uL...

01 November 2016 2,307 3 View

Gel Electrophoresis Analysis

I am extremely confused PLEASE HELP. I watched two videos but I still do not have any clue as to how to interpret my results. Attached are images of the gel electrophoresis. Samples are in this...

29 October 2016 2,954 3 View

Anyone here using the dsDNA 1000 kit for capillary gel electrophoresis of dsDNA fragments?

I'm using the dsDNA 1000 kit as a research tool for some clinic investigations. While the first days all is working correctly as expected, after few days this approach definitely not works more:...

26 October 2016 9,923 2 View

What do I need to start/establish a comet assay experiment in the lab?

I find online kits and equipment specialised for comet assay. My question is: Can't I use a standard gel electrophoresis unit (as the pic) and prepare the buffers for (neutral DSB or alkaline SS...

16 October 2016 6,515 5 View

What could be the cause of peak drifting in capillary electrophoresis?

My allelic ladder has my peaks coming up more than four bases apart to the point that my bins do not catch the peaks. The greatest distance I have had is seven units between the peaks. I have a...

15 October 2016 8,687 2 View

Does anybody have a working protocol for running a native gel for a GPCR?

I have been struggling lately to see expression of endogenous CCR7 in CEM cells as well as exogenous expression 72 hours post transfection of HEK293T cells. No signal (or weak signal) by flow...

30 September 2016 1,682 2 View

Can antibiotics produced in bacterial culture be visualized by native PAGE?

I would appreciate suggestions for other modes of antibiotic visualization, preferably electrophoresis or gel based.

27 September 2016 7,076 4 View

Can mineral (bottled) water be used instead of distilled water for gel electrophoresis?

I have a question that may sound silly. Can meniral water (and by that I mean commercial water purchased with the purpose of drinking) instead of distilled water that is usually used for the...

22 September 2016 2,553 14 View

How to parameterize ligand in MD simulation using VMD-NAMD?

I want to perform MD simulation of protein-ligand complex using VMD-NAMD. When I want to create protein structure file (PSF), there is dialogue box asking me to parameterize my ligand.

15 September 2016 10,018 4 View

Determining protein samples?

Are there any other methods other than gel electrophoresis to determine if protein has been extracted out of the samples?

15 September 2016 5,221 5 View

What program can I use to make a in silico PCR and throw me as result an agarose gel with my expected fragment?

What program can I use to make a in silico PCR and throw me as result an agarose gel with my expected fragment?. Only putting my sequence of interest, my primers and the expected size.

13 September 2016 3,508 1 View

How to count CAG repeats length in a DNA sequence?

Hi, Referring to the above matter, I would like to know the contribution of the CAG repeats length to the risk of developing prostate cancer. In this regards, I am interested in counting...

02 September 2016 6,720 2 View

What may be the effect of voltage variation on 2nd dimensional gel run in Two Dimensional Gel Electrophoresis?

It is well understood that all the samples of an experiment must be processed under identical conditions for comparision among them. If Voltage variation occurs between the samples, when isolating...

15 August 2016 8,877 2 View

How to determine the optimum voltage and time for a better separation in gel electrophoresis?

I am planning to run some of the PCR products on a gel to identify the products of interest, perform a gel extraction, My products of interest will be approximately around 2300bp in size. In...

29 July 2016 4,532 3 View

Anybody have any experience of gel electrophoresis of single stranded lambda-DNA?

I am doing agarose gel electrophoresis of bacteriophage (lambda DNA), and getting inconsistent results for single stranded bands. In my experiment denaturation of lambda DNA is done using 50mM...

25 July 2016 7,137 3 View

Does anybody know any RAPD primer that would yield a good band distribution in a 1.5% gel electrophoresis for Geodermatophilus spp. (Actinobacteria)?

I don't have a pulse-field gel electrophoresis apparatus, so I am using RAPD as a way to qualitatively assess DNA damage after large exposures to UV-C irradiation.

20 July 2016 1,927 2 View

How do I choose the gel running conditions depending on the size of the fragments?

Hi all, I have to run a gel to separate small fragments, 50-25 bp..What are the best running gel conditions (in terms of time and voltage)? I did not understand very well how you choose the time...

12 July 2016 3,097 6 View

I need to purify 45 bp DNA fragment from agarose gel. Does anyone has experience with purifying such small DNA fragment with good yield?

I am looking for a method based on either kit or other.

10 July 2016 2,726 15 View

Non Denaturing Electrophoresis for RNase A, using Sodium Acetate buffer at pH 5, Which gel can be used ? Can anyone share the protocol for this ?

I am trying to separate the various oligomers of RNase A, and want to use the non-denaturing gels for this purpose. Can anyone suggest the type of gel that needs to be used for this purpose. I...

28 June 2016 2,351 1 View

Can any one help me with explaining this gel electrophoresis result?

I had this gel electrophoresis result and I was expecting 480bp amplicon size in lane (2). However, I got a band size of 900bp in (2). Taking into consideration that I did not get a band for the...

27 June 2016 881 7 View

Why my agarose gels drop off in neutralization buffer during alkaline comet assay?

I’m trying to use alkaline comet assay on isolated rat lymphocytes and hepatocytes. But I couldn't get any usable results until now. On the assay, I use high gelling temperature agarose (%0,75)...

22 June 2016 951 12 View

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