Hi All, I need help figuring out what is wrong with my gel images. I don't know the reason for the extra line outside the bright bands. Can anyone suggest a way to avoid that in the future?
We really need to know what the samples are that you are running on the gel. I assume that the extra band is the faint one between the 2 strong bands. It only shows when there is a lot of sample loaded so I think that the solution is to load less sample. It may be that the 2 strong bands are similar in sequence and that the extra band is a heteroduplex of the 2 strong bands which may form better at high sample concentrations
Thanks, Paul. I am talking about the lines that extend from the thick bands, like its outside of the designated well the pDNA samples were loaded into
Thank you Chinenye Izuegbunam now I understand. I can think of 2 possibilities
1 You are using a sophisticated gel viewer capable of measuring the intensity of bands and the line is an indication that the strong band is far too strong to be accurately measurable. Mine used to insert a red band overlying the dna band when overloaded but this might print oddly depending on the optical settings
2 You are using a less good viewer and the overloaded sample is flooding the CCD device and the optics are overwhelmed...a bit like funny effects you get when taking a photograph into the sun. It might be clearer if you ran dilutions 1/2, 1/4 and 1/8 of a strong sample and see if the effect disappears at higher dilutions
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