Gel Electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and... | Contact experts in Gel Electrophoresis to get answers

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Questions related to Gel Electrophoresis

I want to study zymogram of microbial phytase in gel electrophoresis. At least how much amount of broth is needed to prepare ?

I wish to conduct phytase zymogram analysis of few phytate solubilizing microbes isolated previously. I want to know, 1. at least how much broth culture is needed to prepare ? 2. how to...

17 January 2018 5,179 3 View

What are the reasons for this?

In one of the traditional experiments to detect the presence of DNA in some different samples during the study, using Gel Electrophoresis. When tested under UV, this light appears in some...

12 January 2018 1,308 3 View

Forgot to clarify lysate- any consequences?

Hello, I have been preparing lysate for western blot but after i add ripa and sonicate i forgot to run them in centrifuge... I went directly to quantifying protein. I am wondering if this results...

06 January 2018 4,472 4 View

Are these primers OK to give a good RT-PCR result?

Hello everyone, I am master degree student at Mahidol University. I need suggestion from all of you that have the experience in Real-time PCR and/or working lncRNAs. Now, I am working on long...

03 January 2018 6,967 3 View

Can lysozyme can hydrolyze chitosan nanoparticles (for gene delivery) ?

I work on kind of chitosan nanoparticles(Cs-NPs) as gene delivery vectors. I want to find the ability of the plasmid-complexes in preventing DNA migration by gel electrophoresis. sorrily there is...

31 December 2017 4,115 3 View

I am looking for DNA Marker (ladder) for SSR polymorphism test using polyacrylamide gel electrophoresis. Please suggest ?

DNA marker ( Ladder) for microsatellite polymorphic DNA test via poly gel electrophoresis ll

19 December 2017 1,501 3 View

Can any one suggest dna Marker for denaturing gel electrophoresis for 80nucleotide long DNA fragment??

I want to know about DNA Marker which can be used for denaturing gel electrophoresis. My DNA fragment is 80nt long. Please suggest me the company from where I can procure the product?? Can native...

25 November 2017 9,709 1 View

How can I modify a photograph of gel electrophoresis taken with Smarthphone?

Hello all, after perfomed a separition of DNA through the method of Gel Electrophoresis, I can't obtain a photograph of the gel using an UV camera because it was down. So, I used my Smartphone to...

23 November 2017 7,081 5 View

Can I detect amino acids by biuret reagent during separation on agarose gel electrophoresis?

During separation of amino acids using gel electrophoresis, can I use Biuret reagent or Ninhydrin.

15 November 2017 6,676 4 View

My western blots are appearing with streaks?

I am doing western blots for UCP1 in mouse BAT and I have not had problems with previous blots, however, recently all my blots have had the same streaking in the bands. Below are pictures of one...

29 October 2017 9,045 3 View

How do you correctly interpret Gel Electrophoresis results?

Please view the uploaded file, for the Obtain image that i acquired from the completion of the practical.

23 October 2017 1,973 3 View

How to get rid of precipitates in wells in gel electrophoresis?

I am continuously getting some precipitates in pockets in gel electrophoresis. I have tried with incubating the samples at around 40°C for 1hr and also I have tried with low concentration of agar....

23 October 2017 785 3 View

Gel electrophoresis of naked gold nanoparticles, DNA oligo functionalised gold nanoparticles and the DNA oligo used to functionalise the gold?

I thought itd be nice to run a gel contrasting gold nanoparticles, dna functionalised gold nanoparticles and the dna used to functionalise the gold nanoparticles. This would effectively provide 2...

16 October 2017 6,830 6 View

What can I do to refine my fragments for Gibson Assembly?

I'm currently trying to assemble a plasmid with my insert. The plasmid is the SAGFF (6293bp) and my inserts I want to insert (individually) are 2300bp and 3000bp. I have used both a 1:2 and 1:3...

12 September 2017 7,736 5 View

Weird ladder color after transfer to PVDF ?

I wonder if anybody met this situation before.. I ran a gel electrophoresis at 180v for 50 mins and do transfer in 20v 300mA 90mins. after transfering my protein to PVDF membrane, the ladder on...

18 August 2017 1,984 5 View

Anyone use Double-RNA Viral dsRNA Extraction Mini Kit (For Plant Tissue) to extract dsRNA virus particles from yeast?

I am trying to use this commercial dsRNA extraction kit to purify dsRNA virus particles from yeast S. cerevisiae (contains only one dsRNA). But the gel electrophoresis results showed 3 bands,...

22 June 2017 6,021 1 View

Are Ampholytes essential for 2D gel electrophoresis?

Hi, I am working on 2D gel electrophoresis. Is there any substitute of carrier Ampholytes in the rehydration buffer. Is it very essential to run an experiment with 2D gel?

08 May 2017 3,499 6 View

Why my restrictions result isn't visible in gel electrophoresis?

I'm doing RFLP analysis using enzymes RsaI and MnlI (from NEB) for polymorphism in IL-10, but the restrictions results don't show in gel electrophoresis, I've done as the protocol said, 5-15...

03 May 2017 4,172 3 View

Need specific bacterial protien extraction protocol?

After total protien extraction , we will undergo 2D gel electrophoresis . so, the extracted protien must be free of impurities . please help me out guys?

27 March 2017 1,141 3 View

Why i can´t see a good band in electrophoresis gel?

I have problems with electroforessis gel, I made double bacterial transformation and when I want to see two plasmids in electroforessis I only see two Band Spectrum but when i did a normal...

05 March 2017 4,627 3 View

What should i change to improve the quality of polyacrylamide gel?

Hello, I have problem with loading wells in polyacrylamide gel. It looks like the gel around loading wells polimerize poorly. There is a lot of very thin layer gel in the wells after removing the...

27 February 2017 3,440 9 View

I am running a 7.5% Gel but the protein markers are not separated properly. Could you please help me with this?

My reagents are freshly prepared!!!

03 January 2017 9,184 2 View

Why do I get strange band in western blotting?

Lately I ve been having a problem with electrophoresis of western blotting, I run 120v for 2h, i got strange band like picture. I used fresh buffer.

27 December 2016 6,490 7 View

Why there is a differences in the results obtained for DNA Quantification with micro spectrometer and gel electrophoresis?

Hi friends, let me explain my query in detail. By analysing a ladder standard on Micro spectrometer I am getting answer 505ng/ul, which is quite correct, the standard is of 500ng/ul. Similarly, I...

12 December 2016 3,967 2 View

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