23 December 2019 3 838 Report

Hi all,

I have been trying to do cloning recently. After lots of effort, I finally come to do the mini prep with iNTRON kit. When we shake the colony in 5 ml of LB with 5ul of ampicillin. There was bacterial in the tube and we could also measured the concentration (~300ng/ul) of the plasmid with normal A260/280= 2.13 and normal 260/230. However, when we load 1ul or 2ul of the plasmid to run a 1% agarose gel, no bands were seen. How could this happen?

Thank you so much!

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