How does a wrong (higher) concentration of loading buffer affect the results of a gel electrophoresis?

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Paul Rutland

I would expect smiley bands early on in the separation straightening out as the salt moves away from your dna sample later in the electrophoresis

Mantu Kumar

I don't think it will affect the migration. possibly you will observe the dye, much denser than normal (1X) in the gel while electrophoresis.

Ali Javadmanesh

You won't see any problems in Agarose electrophoresis! There night be a minor issue with SDS-PAGE as Paul Rutland mentioned.

Viola Lalomia

Thank you very much for your answers!

Amy Johnson

Ali Javadmanesh Mantu Kumar Paul Rutland