Flow Cytometry

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell... | Contact experts in Flow Cytometry to get answers

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Questions related to Flow Cytometry

How to encounter endogenous bacterial contamination?

Dear fellows I am into micropropagation of calladium. we didn't observe any bacterial contamination during the initiation and first subculture stages. the second subculture was done last week and...

10 August 2022 6,352 3 View

I am a researcher on chromosome segmentation of g- banded metaphase images. I want to know the segmentation approaches based on Deeplearning?

Currently there have been a trend of using improved versions of Unet of CNN architecture. I would like to know the current status

17 June 2022 2,209 0 View

What is a good antibody to identify endothelial cells and differentiate from glia?

WE use ZO-1 - but see weak signal with hbec51 cells

08 February 2022 2,437 2 View

Where can I find spare/second hand BD Accuri C6 flow cytometer parts?

Hi all, We recently acquired a second hand Accuri C6 flow cytometer (manufactured 2012). Production and support of this model has been discontinued by BD following supersedence by the C6+. We're...

22 December 2021 7,860 2 View

BD Buffer substitute?

I am working with an antibody panel for immune-profiling using flow cytometry and I am looking for a substitute for BD's polymer-based staining buffer. I know that for western blotting a blocking...

16 November 2021 7,203 2 View

Can you get H2O2 by WOR followed by ORR?

We are testing our photocatalyst for both water oxidation reaction (WOR) to O2 and oxygen reduction reaction (ORR) to H2O2. Can you in principle close the cycle from H2O to H2O2 (and then back to...

24 September 2021 3,560 3 View

Stains (flow cytometry) needed for isolated red blood cells?

I have red blood cells isolated according to the protocol attached. I would like to know what stain would be required for flow cytometry to identify the red blood cells for gating. Also, if I want...

17 September 2021 1,850 1 View

How will you prepare the N-acetylcysteine (NAC) concentration?

Dear all, I would like to use N-acetylcysteine (NAC) to rescue the ROS-inudced cell death caused by a drug treatment. However, I found the final concentration of NAC people used widely ranged...

09 September 2021 4,946 4 View

How to perform success Intracellular IL-4 staining in immune cells by flow cytometry?

I am trying to stain cytokine IL-4 in dendritic cells and Th2 lymphocytes recently. I am using a standard intracellular cytokine staining (ICS) protocol. Briefly, cells were stimulated (10ng/ml...

05 September 2021 846 2 View

Is fixation needed for staining epigenetic modificaitons by intracellular flow?

I was trying to measure the level of h3k79 methylation through intracellular flow. Basically, all the intracellular flow protocols include a fixation process, but since epigenetic marks are...

31 August 2021 5,793 0 View

CRISPR technology vs Restriction Enzymes . What are the major differences ?

How is CRISPR technology a better option when compared to Restriction Enzymes , when it comes to the point of cutting a part of a DNA ? How safe and beneficial is it when it comes to the point of...

04 August 2021 5,547 4 View

How to disaggregate the clot that results from aggregation of pbmcs in buffy coat ?

When i am isolating pbmcs, iam getting a white clot (aggregated PBMCs) in the buffy coat, so I can see just a few number of separated pbmc cells, and this problem conflicts with counting of pbmc...

03 August 2021 9,954 4 View

What is the procedure of RBC counting using flow cytommetry from a whole blood sample?

When an anti-coagulated blood sample is taken, how can we calculate the number of RBC, WBC, and platelets using the flow cytometry technique? Do we need to separate the components in the blood...

05 July 2021 168 5 View

For how long can I stock Anhydrotetracycline in DMSO?

Hi everyone, I'm currently working with anhydrotetracycline solution in DMSO and I couldn't find for how long I can use the same aliquot and how should I stock it. Does anyone can help me? Thanks

28 June 2021 5,029 3 View

Can PGE1 have any effect on activated platelets?

Hello. I am testing PGE1 on my washed platelets, which is suppose to inhibit platelet activation. If the platelets have been left at room temperature for a while, and possibly activated, in this...

25 June 2021 7,506 1 View

I am growing NIH3T3 cells, would highly appreciate if you can figure what is the problem in it?

I am growing NIH3T3 cells and i have tried so much but cant get 70-80% confluence. After 24 hours the medium gets turbid. Also there is a lipid like layer attached to the wall. if you can please...

24 June 2021 436 4 View

Is there a way to find the reference paper based on a sentence or a paragraph from the main text?

I copied a paragraph from a academic paper and saved in a word document a few months ago. However, I forgot to leave the reference at that time, so I want to find the reference of that paragraph....

17 June 2021 8,809 5 View

Has anybody performed an indirect staining for flow cytometry?

Hello! Besides through IF, we want to detect an intracellular recombinant protein through flow cytometry. However, we don´t have a conjugated antibody against it, so we plan to detect it through...

04 June 2021 7,519 2 View

Can I use cells stored in RNAlater directly for RNA isolation?

I'm planning to isolate RNA from cells stored in RNAlater with Qiagen mini kit. In one article, I saw we can directly use cells in RNAlater without washing it with PBS (but needs to put a higher...

25 May 2021 921 3 View

Protien elution using sep pak ?

Hi everyone, I’d asking about the elution step of protien in sep pak c18 , this is the first time that I’ve made the elution with acetonitrile , the question is when the elution process done the...

24 May 2021 8,700 6 View

Th17 cells differentiation in vitro?

Hi all, I am curious that why in some methods, people add IL1b and IL23 on top of the TGFB and IL6 for Th17 cells differentiation in vitro? Also, the percentage of Th17 after in vitro...

19 May 2021 769 4 View

The objective of the experiment is to detect the number and percentage of Th17 cells and Treg in the gums of mice. What method is recommended?

The objective of the experiment is to detect the number and percentage of Th17 cells and Treg in the gums of mice. What method is recommended? Flow cytometry or immunofluorescence? If flow...

06 May 2021 6,901 2 View

How to put Overlay histogram in Flowing software with different peak height?

Dear All, I have a doubt regarding flowing software.If anyone using the software kindly help me. My problem is while combining all the individual histograms, peak height of each individual one...

23 April 2021 9,287 3 View

Oil red O protocol for flow cytometry?

Hello, I´m analyzing mesenchymal stem cells from fat in flow cytometry, but I need to assure that I am selecting the population of interest, and not adipocytes. We have used oil red in slices for...

19 February 2021 4,461 3 View

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