I am trying to characterize the foreign body reaction surrounding my implant, and all the cells are stained positive for CD31. I am not sure if they should be! (I am implanting my material in the anterior chamber of the eye.)
I am also staining for alpha-SMA, and that's almost ok.
Any insight will be very helpful.
The short answer is "yes". CD31 (PECAM1) is present on foreign body giant cells/multinucleated giant cells/histiocytes.
https://academic.oup.com/ajcp/article/143/2/177/1766537
Article Expression of CD34 and CD31 in Central and Peripheral Giant ...
Article Lack of Platelet Endothelial Cell Adhesion Molecule-1 Attenu...
Thanks Ellen A G Chernoff ! I also read about PECAM-1 being expressed on most cells of the hematopoietic lineage, including monocytes. However, this protein is also highly expressed on endothelial cells. I was looking for other publications where they have an implant and show vascularization. But all I could see was images of CD31+ regions that indicate vascularization and NO foreign body reaction, for example this one: (https://link.springer.com/content/pdf/10.1023/A:1023822504224.pdf).
I am also interested in showing vascularization in my implant but the fact that everything stains + for PECAM makes things hard to understand. Maybe I have to pick another (more specific) endothelial cell marker.
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