Can't generate a mash for topology in ANSYS. how to do it?

Created model of steel caisson in space claim. Used a static structure for topology. But there's an error "one or more surfaces cannot be mashed with acceptable quality", " One or more entities...

25 January 2021 893 1 View

Do you know any country-specific methodologies of urban public spaces evaluation?

Hello! Currently I am working on a research about different methodologies of urban public spaces evaluation/their impact assessment. The only comprehensive projects with explicit measurement...

18 November 2020 2,428 5 View

How to understand question about epistemological starting points and theory on one side and method on the other?

The whole question looks like that: Discuss the relation between epistemological starting points and theory on one side and method on the other, concerning two methods (or two methodological...

31 October 2020 2,580 6 View

EPR: What to do if there is no hyperfine?

What are the most common factors besides clustering and spin-spin interactions of paramagnetic atoms that lead to poor spectra without expected hyperfine?

19 March 2019 9,885 2 View

Is there a way to coat latex beads with a ROS scavenger such as sodium pyruvate?

Does anyone know if it is it possible to coat 1um latex beads with a ROS scavenger such as sodium pyruvate? Do the beads need to be modified? Thank you!

11 March 2018 6,726 3 View

Liposome delivery

Hello everyone, I am looking to see if it is possible to encapsulate soluble bacterial peptidoglycan in a liposome. Are there any kits out there I could use? They seem to be mostly for DNA/RNA...

17 May 2017 4,967 3 View

What analysis and what software should I use if I want to model how one censored variable predicts another censored variable?

My research has to do with employment and reincarceration after release. I have used cox proportional hazard model to see whether participating in vocational education while in prison affects the...

25 January 2017 8,624 2 View

Does anyone know a method (or commercially available kit) for simultaneous isolation of plasmid DNA and total RNA from the same sample?

28 August 2016 2,145 3 View

What is the history of pop-up areas? Is there are any theoretical information?

I need to know more about history of pop up places (shops, restaurant and so on), but from the theoretical side

18 May 2016 9,297 2 View

Why do my cell not adhere to the culture dish after?

22 December 2014 4,513 10 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View