If I prepare a stock of undiluted platelet rich plasma from whole blood, I then would like to perform a count on a flow cytometer. I take a small sample of e.g. 20uL and flow cytometer tells me e.g. x events/uL in the 20uL sample.
Now I would like to find out how many events were in the original undiluted stock per mL.
Do I multiple my x value by 1000 to find the platelets/mL in the original stock or this is too simple of an assumption?
I frequently do this exact process to count and extrapolate counts of individual immune cell types from peripheral blood. I always lose some cells in spin downs and in staining, but this is generally about the same across the board, so differences between treatment groups still hold true. This is slightly less accurate than counts that I get from using a hemocytometer, but it is still a very good way of quickly counting the cells for a number of samples.
I hope that this helps and good luck with your experiments!
Hello Rh Rj
,The calculations that you listed in your comment (find the events/uL iand then multiply by 1000 to find the events/mL) are correct to convert from cells/ul to cells/ml in the original stock.
I am glad that my comments were helpful and good luck with everything!
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