Hi!
I want to stain embrioid bodies that I have in culture using immunocytochemistry method.
I also want EBs not to completely flatten out on the plate - it would be perfect if they'd remain 3D structure (even partially).
CD34 protein would be stained to see if we obtained HPCs cells. Unfortunately, I can't use flow cytometry, therefore I'm trying to analyse cells using other methods than FC.
Is it possible to attach EBs to the plate using Geltrex/laminin etc.?
Have you ever perfermed such staining and was able to stain markers typical for HPCs cells?
Thank you for any advice!
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