Gaussian Optimization Resulting in Unexpected Bend in Structure?

I have been trying to optimize a structure utilizing the ONIOM method of optimization with high level ground state MP2 6-31G d,p and low level ground state 3-21 settings. However, the optimized...

08 February 2020 2,599 2 View

How to make Dragon 7 software detect aromatic structures?

I have a problem while calculating molecular descriptors with Dragon 7. My structures are drawn in Avogadro and optimized with Gaussian. After this step, my structures look like the one I uploaded...

18 January 2020 934 1 View

Is GM the answer?

How can GM technologies serve enough food for the human population which is growing rapidly every year and if we compare with sociocultural aspect of human, poverty and planting areas? Maybe we...

15 December 2018 1,874 1 View

Does anyone have a good reference for using QQ-plots for imputing left censored data?

26 June 2016 1,877 6 View

Is the Thermal Insulation of Buildings Misbelief or Answer of the Problem?

25 August 2014 947 7 View

Does anyone know of new articles that discuss the peer review process?

I have been trying to develop guidance for my group for doing thorough peer reviews. Would like to get some input from other groups

05 March 2013 7,314 13 View

What's about Bronze Age amber ?

01 January 1970 1,768 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View