I have no nearby mouse facility and cannot bring live mice to my research facility. I therefore have to transport either the euthanized pregnant mouse or the mouse embryos (staged E11.5-E14.5) for 1 hour before even starting the dissection. The embryos are used for explant culture of inner organs, so I want to keep the cells alive.
Does anyone have experience with a good method? I'm considering:
1) Transporting the intact (euthanized) pregnant mouse on wet ice
2) Dissecting out the uterus and transporting it in a tube of PBS OR culture medium on wet ice
3) Dissecting out the embryos and transporting them in a tube of PBS OR culture medium on wet ice
As a general tissue culture viability guideline, the smaller the mass of tissue transported the better. This will allow maintenance of pH, nutrient access, gas exchange. If you cannot isolate organs at the mouse facility, I suggest whole embryos in gassed DMEM/F12 (bubble O2/CO2 incubator mix) on ice.
u can bring the embryos in HANK'S balanced salt solution in normothermic condition. never impose them to too cold condition.
For live embryo to bring to your lab you can used hypothermic preservation media having tissue culture medium 199 supplemented with 50% fetal bovine serum and 25 mM HEPES. We have used this media for bovine embryo and even at 4 degree temprature the viability rate of embryo was 100% for 24 hours and 48 hours, for refrence you can referre to 1-s2.0-S0093691X2030265X-main (1).pdf as well as the origional protocole publish is scientific reperots (Scopus - Document details - A simple medium enables bovine embryos to be held for seven days at 4°C).
you should bring live pregnant mice to lab and euthanize in your lab. That is the correct way
The point of my question was that transporting live mice is not an option. It's not allowed due to national law.
Imran Khans tranport medium worked well for transporting whole E12.5 mouse embryos at 12C for 45 minutes. They made fine organ explants that grew as normal. So thanks Imran Khan!
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