Flow Cytometry

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell... | Contact experts in Flow Cytometry to get answers

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Questions related to Flow Cytometry

Why cell populations meshed together in apoptosis analysis using Annexin V-APC/7-AAD?

Hi all, I'm doing apoptosis analysis using Annexin V-APC/7-AAD on a Beckman Cytoflex flow cytometer. The band pass for APC is 660/20, and for 7-AAD it is 690/50. Laser delay for 488nm laser is 0,...

10 October 2019 9,365 5 View

Which are the best combinations of human FoxP3 antibody clones for optimum & specific detection of Tregs by flow cytometry?

Using two clones against human FoxP3 in combination is supposed to increase sensitivity for detection of these cells ( Association of Two Clones Allows for Optimal Detection of Human FOXP3 )...

10 October 2019 383 2 View

Streptavidin APC vs Streptavidin PE for cell sorting?

I am trying to sort cells based on a biotnylated-protein tetramer. After biotinylation I have to option to bind my protein to S-APC or S-PE beads in the protocol and wanted to know if there are...

10 October 2019 1,303 3 View

Do all tumor cells express MHC-1 protein that can be detected by Flow Cytometry?

I understand that Antigen Presenting Cells (APCs) like tumor cells express MHC-1 which in most cases might be altered. Before starting my main experiment, I did a preliminary experiment to...

10 October 2019 1,180 4 View

How to do T cell suppression assay (mouse) ?

Hi, everyone! I tried to do mouse MDSC and T cell coculture assay, but failed. I have some questions. 1.Bone marrow derived MDSC can inhibit non-specific activated CD8+ T cell or not? (CD3 and...

10 October 2019 785 1 View

What is the percentage of microglia in the healthy rat brain measured by flow cytometry ?

I measured microglia population by flow cytometry using a combination of CD45/CD11b,c antibodies in the healthy rat brain. Microglia population identified as CD11b,c+/CD45low cells represent 0.2%...

25 September 2019 7,711 2 View

PBMC and MSC fixation for flow analysis?

Good Day: I am looking for a protocol for fixation of PBMC's and MSC's (Human) that will allow for surface analysis by flow cytometry. Thx and best regards...dwf

25 September 2019 9,480 3 View

Applying an old compensation matrix to dataset ?

Hello, I did some flow cytometry acquisition a week ago. Now, I finally got the chance to analyze the data and I unfortunately discovered that one of my compensation controls didn't work well:...

23 September 2019 6,239 4 View

How to identify blood macrophages?

Hello, What is the best way to detect and identify macrophages in blood samples? I guess using flow cytometry with labelled-antibodies targeting macrophages M1 and/or M2 analyses could be a good...

20 September 2019 5,500 4 View

Th17 differentiation problem ?

Dear all, can sombody give me some suggests, I do not why I can not detect IL17A Expression, I really do not know what is wrong with my differentiation, please give me some advise. I isolated...

20 September 2019 5,972 3 View

Applying an old compensation matrix to dataset ?

Hello, I did some flow cytometry acquisition a week ago. Now, I finally got the chance to analyze the data and I unfortunately discovered that one of my compensation controls didn't work well:...

09 September 2019 9,680 4 View

What is the percentage of microglia in the healthy rat brain measured by flow cytometry ?

I measured microglia population by flow cytometry using a combination of CD45/CD11b,c antibodies in the healthy rat brain. Microglia population identified as CD11b,c+/CD45low cells represent 0.2%...

09 September 2019 3,970 2 View

How to identify blood macrophages?

Hello, What is the best way to detect and identify macrophages in blood samples? I guess using flow cytometry with labelled-antibodies targeting macrophages M1 and/or M2 analyses could be a good...

09 September 2019 6,977 5 View

PBMC and MSC fixation for flow analysis?

Good Day: I am looking for a protocol for fixation of PBMC's and MSC's (Human) that will allow for surface analysis by flow cytometry. Thx and best regards...dwf

09 September 2019 6,416 3 View

Markers for murine gamma delta T cells?

Any specific markers for murine gamma delta T cells for Flow Cytometry??

09 September 2019 4,700 4 View

What is the best method to quantify the Treg membrane receptors?

I am considering flow cytometry and PCR. Are these methods not gold standard for surface receptor quantification? I have been suggest to perform western blot.

09 September 2019 5,265 3 View

Does anyone know what the wash buffers (A and B) and Detection Stock are in Duolink kit?

We want to use Duolink by flow cytometry. We have the kit suitable for fluorescence, PLA probes, and primary antibodies, but we don’t have the wash buffers (A and B) and Detection Stock (5x). Is...

09 September 2019 8,549 1 View

How do I unmix GFP Signal and yeast autofluorescence?

Hello everybody, can someone give me a hint how to unmix fluorescence data in GFP and autofluorescence from yeast cells in flow cytometry? Thanks a lot in advance

09 September 2019 6,441 2 View

Determining Fungal growth in a complex medium with bacteria?

Hi Everyone, For my current project, I am growing bacteria and fungi together to mimic the gut microbiota (cultured from faeces). I am looking for a protocol to evaluate fungal growth from a...

09 September 2019 6,134 1 View

How to get Th17 cell clone?

Hello everyone, Do you have experience in the isolation of Th17 cell clone from human PBMC? One common method is to firstly isolate naive CD4+T cells, then to use flow cytometry sorting (CD161...

09 September 2019 4,636 7 View

How to get Th17 cell clone?

Hello everyone, Do you have experience in the isolation of Th17 cell clone from human PBMC? One common method is to firstly isolate naive CD4+T cells, then to use flow cytometry sorting (CD161...

06 September 2019 8,124 7 View

What is the effect of Ca and Mg to the protein-protein interaction?

I am studying an interaction between 2 proteins (both of them have Ig domains). I am trying to prove their interaction by staining cells which express the protein A with the biotylnated ECD (extra...

29 August 2019 6,843 1 View

Are viral diseases taking upperhand over bacterial, parasitic and fungal diseases in the world?

Earlier times country like Bangladesh was busy with bacterial diseases like TB, Cholera, Typhoid etc, parasitic diseases like Malaria and Kala azar or fungal diseases like Candida. But for recent...

15 August 2019 5,791 15 View

Should I show FL+ % or Statistical data for my FACS analysis when measuring phosphorylation levels in treated cells?

I treated cells with certain kinase inhibitors (including Vehicle) and measured phosphorylation levels (single stains with specific primary ab and AlexaFluor488) with FACS. I analyzed my data in...

14 August 2019 5,209 3 View

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