What are the steps for microbiological test validation, namely the total yeast and mold test?

More Mona Indriani's questions See All

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,291 3 View

How the ventilator generates positive pressure in PSV?

I read that "PSV assists the patient's effort by delivering a positive pressure during inspiration. This reduces the work required to expand the lungs and overcome airway resistance, making...

20 April 2024 1,660 1 View

How to increase the efficiency of doxorubicin entrapment in albumin nanocarrier?

I obtained up to 30% encapsulation efficiency by using the desolvation synthesis method, in different pH level(7-8/5) with 8% glutaraldehyde as a cross-linker, this work has been repeated many...

19 April 2024 8,496 3 View

Is rhodamine B can be used to prepare chlorophyll ‘a’ concentration?

I want to measure spectra of Chlorophyll ‘a’ in the lab , Is rhodamine B can be used to prepare chlorophyll ‘a’ concentration with the aim to measure spectra in lab using spectrophotometer?

14 April 2024 7,550 0 View

Why reflectance is greater than 1 in some cases?

I prepared different concentrations of chl-a using Rhodamine B (CHClN₂O₃) as indicator, my aim is to measure the spectra for each concentration trying to find relationship between them, the...

19 March 2024 2,147 0 View

what the applications of Tag SNPs selection and Haplotype Blocks are in the context of rare pathogenic variations?

Hi, Could somebody tell me what the applications of Tag SNPs selection and Haplotype Blocks are in the context of rare pathogenic variations in a Mendelian disease. Appreciate your...

17 March 2024 4,034 0 View

What are the best HW p-value & MAF cutoffs based on my study ?

Hello, Based on my research question, I would like not to exclude variations that are out of Hardy-Weinberg equilibrium, and I also want to have rare variants in my study ( i mean the MAF...

07 March 2024 5,788 0 View

How display the SNPs positions along a chromosome on the top of my LD plot which provided by Haploview?

Hi, I have provided my LD plot by Haploview and I used Linkage Format (.ped & .info) to this aim. I am wondering how could I have the gene structure on the top of my LD plot ? I mean I need...

06 March 2024 5,150 2 View

Is this a bacillus subtilis colony? on Hicrome bacillus agar medium?

I bought Bacillus subtilis ATCC 6633 bacteria, I grew them on Hichrome bacillus Agar media: green colonies with white crincle or wavy edges and the media changed from pink to yellowish. Is this...

01 February 2024 6,901 0 View

What is the pre treatment of HZSM-5 for BET test?

Hi everyone I want to analyze the HZSM-5 by BET but I don`t have any information about how to degass it in which condition ( temperature and duration)

04 November 2023 9,936 1 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,822 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,731 2 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

What is fitofthora instance of potatoes?

Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.

02 August 2024 179 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is gangrine? Is there gangrine happened indie potato?

Please discuss someone told about fungus and allied others.

31 July 2024 3,372 3 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

Help me to identify these fungal slides?

These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..

13 July 2024 9,701 1 View

Has anyone calculated the effective concentration at 50% (EC50) value of different fungicides for the fungus Tilletia horrida?

How can we measure their colony diameter?

12 July 2024 7,027 0 View

I am carrying out an experiment on Trichoderma. How can I measure OD of the fungal growth after 7 days?

I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....

11 July 2024 6,250 1 View

Phil Geis

What is context - drug, cosmetic, food?

"Validating" - as in validating recovery in presence of product?

Francesc Codony

You can find different approaches for validating microbiological methods in ISO 16140 standards.

Mona Indriani

validation on drug samples. is it only enough with a spike sample representing 1 yeast and 1 mold. or how many microbes should I use @Phil Geis

thanks for all your answers Francesc Codony

Yes - Candida albicans and Aspergillus brasiiensis in "suitability" testing - but use the strains and methods specific in USP's 61/62. Do not use ISO 16140 - that is for foods.

https://www.usp.org/sites/default/files/usp/document/harmonization/gen-method/q05b_pf_ira_34_6_2008.pdf

https://www.uspnf.com/sites/default/files/usp_pdf/EN/USPNF/generalChapter62.pdf