For LOD and LOQ
I need a *blank sample* and *sample of known concentration*.
For the *blank sample*, should I just fill the vial with my diluent only ?
And for *sample of known concentration*, should I use my concentration of my analyte from method development?
For determining the LOQ of a HPLC peak you need a blank sample and a regression equation. The LOQ is defined as the concentration you obtain by putting 10 times the signal intensity of the blank sample into the regression equation. For this you need to inject a blank sample and several samples of known concentration to build the regression equation.
You need blank sample containing your Diluent only, to observe if there is any other peak other than your Std.
for LOD and LOQ, you can use sample of known conc.
There are two methods to determine LOD, LOQ
1. by making the range of serial dilutions, plotting the calibration curve and analyze by data analysis in MS Excel.
2. by making and inject the lowest possible concentrations of Std to be analyzed and check the conc. limit, where peak response is enough detectable.
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