22 May 2024 1 4K Report

I am looking for the optimized protocol for protoplast isolation by using just expanded unifoliate leaf of Mung Bean.

More Dipak Khanal's questions See All
Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,604 2 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 266 3 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,216 4 View

Leaf area of tomato ?

Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of ​​a tomato? Can you explain with an example and what is the substitution of Ln and ln?

06 August 2024 2,508 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,802 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,786 4 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,018 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,724 2 View

What are the limitations and challenges of using machine learning for predicting concrete compressive strength in practical applications?

Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...

03 August 2024 2,545 2 View

Similar questions and discussions
Hello Researchers, Can anyone guide me on estimating the Leaf Area Index (LAI) of wheat using only weather and soil parameters? Is it possible?

I need to estimate the LAI of a wheat crop using only weather and soil parameters. Can anyone please help me?

28 July 2024 4,884 2 View

Can we preserve the protoplasts?

Can we preserve the isolated protoplasts for future experiments? Is it possible to preserve them via cryopreservation? If yes how long can we preserve them?

16 July 2024 9,576 1 View

Any homemade protocol to extract genomic DNA from leaf litter?

We are trying to extract DNA from ant nests to amplify the 16S gene, but apparently, the extraction allows the passage of PCR inhibitors. Thank you for your comments

15 July 2024 9,314 2 View

Hello friends In simulating the drug with the liposome, which side of the liposome membrane is the drug placed on? The top leaf or the other?

Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...

29 June 2024 9,687 1 View

What is the best part of a leaf to study the anatomy of monocot and dicot plants?

The first 1/3 of the leaf (near the stem), the middle 1/3 of the leaf, the last 1/3 of the leaf Monocot: Corn Dicot: bean

28 June 2024 2,606 4 View

Collaboration with researchers from different countries asking for the morphometric data based on herbarium samples?

Dear Colleagues: Good day and greetings from CSMCRI, India! I am thankful to all those who have responded to my previous query posted in the list last year. We are pleased to inform that our work...

19 June 2024 2,467 0 View

Hello all I am currently working in extracting plant parts using ultrasound. Can I use the GC-MS technology or chromatographic analysis?

A number of active compounds and their concentrations found in flower and leaf extracts were obtained using this technique

09 June 2024 1,871 4 View

How to use LAMINA - Leaf shApe deterMINAtion software?

Suggestion needed on using LAMINA - Leaf shApe deterMINAtion sotware.

05 June 2024 9,105 0 View

What is the suitable concentration of hygromycin in tomato sp. Micro-tom genetic transformation?

I used hygromycin and kanamycin for selecting positive buds in Micro-tom genetic transformation. Kanamycin performed very well and I could get positive plants soon, but hygromycin is a little bit...

29 May 2024 6,911 0 View

What are the effect on nodulation in mungbean by using selective herbicides? What herbicide might be best to minimize impact on root nodulation?

hand weeding is time consuming, costly and other mechanical weeding practices are not available here. I am seeking for effect of selective herbicides on root nodulation in mung bean farming....

22 May 2024 4,141 0 View

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