I was hoping anyone could provide any insight into using ATCC's "RD-ES" and "A-673" cell lines. How many passages do you do before you notice any senescence or genetic abnormalities
These are tumor cell lines, under proper cultivation conditions they will not undergo senescence. The cell lines have been propagated for many passages, if they are further mutating you can only determine by WES, WGS and for copy number alterations also by SNP array analysis.
Hello Daniela Cox Beltran
There is no defined maximum number of passages you should perform for a cell line, but it is highly recommended to keep passage number low. Low-passage cancer cell lines are generally preferred due to their closer resemblance to the original tumor.
Whenever I have worked on cell-based assays using cancer cell lines (not worked with RD-ES and A-673 cell lines), I usually use cancer cells until passage 20 and do not go beyond passage 25 to maintain consistency and reliability of results.
This may not be the answer to your question, but it may give you an idea how far you could go passaging the Ewing sarcoma cell lines before observing any senescence or genetic abnormalities.
Best.
When you obtain a cell line from a repository, usually there is no indication of the passage number, so you may only record your own passages, and as mentioned, under appropriate culture conditions tumor cell lines will not undergo senescence.
Hi Daniela Cox Beltran
Because of selective pressures and genetic drift, cell lines, when kept in culture too long, exhibit reduced or altered key functions and often no longer represent reliable models of their original source material.
For more information, you may be interested in the below attached paper.
Article The Costs of using Unauthenticated, Over-Passaged Cell Lines...
Best.
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