What if top fiber concrete strain exceeds the ultimate strain when fixing the yield strain in steel i.e to calculate yield moment in circular column?

I have prepared an excel sheet to calculate yield moment and curvature for a hollow circular section. In which Depth of Neutral Axis is fixed using "GOAK SEEK" function. Axial Force is input of...

22 November 2020 4,632 3 View

How to do MD simulation of docked protein complex after docking .?

I have perform some protein-protein docking studies. I used Hdock, Prizm. But I am unable to find a proper method to verify my docked structure. I also docked my structure in Hex program to get...

19 October 2020 4,083 7 View

I want to know about command to fetch XTC and PDB file For markov Calculation?

I am learning markov state model in jupyter notebook. When I am fetching data with mdshare.fetch() command from tutorial its okay. But how I will fetch XTC and PDB File from my local source. Which...

10 August 2020 5,379 3 View

How to fix error of GROMACS topol.top file?

I am doing MD simulations for protein. When i am running the commands the following error is occurred Command which i have used with ubuntu gmx genion -s ions.tpr -o solv_ions.gro -p topol.top...

27 July 2020 3,199 15 View

What is exact method to measure PL emission of traditional MAPbI3 perovskite film on the glass subsrate?

I am preparing film on glass from the commercial materials following reported protocol. UV-vis absorbance is getting perfect results according to literature but PL response is not achieved in...

12 April 2020 127 9 View

Which is the best ELISA assay kit for the measurement of mouse serum and urine creatinine?

Hi, I am looking mouse ELISA kit for the evaluation of creatinine level in serum and urine. Could you please suggest me which kit is most reliable for this assay? Regards, Nirmal

03 December 2019 824 4 View

How to Manufacture the Nano Powder of Zirconium or Zirconia from Micron Size available in market?

Zirconia or Zirconium NaNo Powder Production

26 November 2019 2,247 3 View

Can someone Help me out with a suitable UDF to import variable properties of SUPERCRITICAL Water?

I am not able to hook up my table of ISOBARIC (225 Bar) PROPERTIES OF SUPERCRITICAL WATER (varying wrt Temp) obtained from NIST into ANSYS FLUENT which only accepts a C Code, Piecewise Linear and...

27 October 2019 4,424 5 View

How can I convert charmm parameter and toplogy file in to the amber format?

Hi I am following a paper "Development of CHARMM-Compatible Force-Field Parameters for Cobalamin and Related Cofactors from Quantum Mechanical Calculations". In this paper they used charmm force...

25 September 2019 8,059 7 View

Can the capacitance of a Graphene based FET can be calculate via CV charge discharge method?

As in case of capacitance calculation for super-capacitor the CV charge discharge method is popularly used. Can the similar method be implement to calculate the capacitance for graphene based...

27 August 2019 2,843 5 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?

Hello, I have performed two westerns in parallel, one using fresh buffer (made with old 10X tris-glycine) and the SDS gel was made 4 days prior to western; and the other western was performed...

03 February 2021 9,311 5 View

How to measure volume of Pinus roxburghii ?

I want to measure volume of Pinus roxburghii in the Jammu region of India to calculate the carbon stocks of Pinus roxburghii.

31 January 2021 2,311 3 View

What are the factors on which exchange current density of an electrode depends, and how can they be predicted?

While surface roughness is an obvious factor, this is not what is exactly asked. Rather, periodic variation of exchange current density (of an electrode) with solution chemistry (of electrolyte)...

30 January 2021 4,886 5 View