How to dilute my DNA Template( 1 Micromolar stock solution ) into1 × 10-8 nM to 1 × 10-15 nM ?

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Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

How is artificial intelligence being utilized to enhance the diagnosis and treatment of sleep apnea?

AI has the potential to improve the management of sleep apnea by personalizing treatment, enhancing diagnostic accuracy, and advancing our understanding of the condition.

03 July 2024 9,393 2 View

Why can't Ansys HFSS be used for LiDAR Based Simulation?

Hey All, I am currently trying to simulate an FMCW Lidar in Ansys HFSS which I used for RADAR Simulation and I cannot find a way out to perform Lidar based simulation. If anybody knows how to do...

20 March 2024 2,749 2 View

Hi, A phospholipid qualitative analysis is to be performed in liposomal formultations.Is HPLS-UV a reliable technique for same ?

A phospholipid content has to be estimated in liposomal formulations.Is HPLC-UV a reliable technique for same or should other techniques be employed?Which one is the most suitable of them all ?

22 November 2023 4,883 1 View

How to generate ligand.itp and ligand.gro files for md simulation using amber14 force field?

How to generate ligand.itp and ligand.gro files for md simulation using amber14 force field?? Kindly help!! Thanks in advance.

12 November 2023 2,434 4 View

Where I can do extrusion of Magnesium alloy in india?

Any vendor or Institute.

05 November 2023 3,030 0 View

Error while generating ligand topology?

I'm trying to generate ligand topology files through CHARMM FF but i'm getting this error. I installed the respective python version but error is still same python cgenff_charmm2gmx.py UNK...

23 October 2023 1,587 4 View

How can I insert a protein structure model within Lipid bilayer structure model?

How can I insert a protein structure model within the Lipid bilayer structure model? Kindly help.

12 May 2023 5,082 3 View

What are the alternate servers for PRODRG to generate ligand topology? I have tried ATB, ACPYPE also but ligand gets detached from the protein?

what are the alternate servers for PRODRG to generate ligand topology? I have tried ATB, ACPYPE also but ligand gets detached from the protein while generation complex.gro file in the MD run....

23 April 2023 7,765 3 View

Do we need to add TGF-beta into fibroblasts obtained from idiopathic pulmonary fibrosis patients to promote EMT?

I purchased the cell line LL29 from the ATCC a while ago. This cell line is isolated from the fibrotic lung of the IPF patients. I believe this cell line is already lung fibrosis disease cell...

20 April 2023 2,553 0 View

Methyl jasmonate preparation ?

How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?

01 August 2024 723 0 View

Shortage Cost Hypothesis?

What can be a hypothesis related to the Stock out Cost or Shortage Cost?

29 July 2024 9,259 2 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View

How do you get pure primary macrophage cell culture?

I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...

23 July 2024 4,074 2 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

How to prevent microbubbles during immunofluorescence imaging?

Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...

01 July 2024 7,694 4 View

How to make 100ppm 1Liter methylene blue stock solution? How much weigh the dye require?

methylene blue stock solution

19 June 2024 10,030 3 View

What are impact of stagflation on emerging markets performance?

The emerging markets have suffered from a state of stagflation. I hope you know your expectations according to your experiences regarding the implications for the stock markets,,,, risks and...

19 June 2024 4,410 3 View

Why is iPSC in bad condition?

I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...

12 June 2024 6,087 0 View

How to Dilute Stock solution of ferrous sulphate in frap assay?

I want to do Frap assay with ferrous sulphate heptahydrate (FeSO4.7H2O) as the standard. I have calculate the molarity which is 1 M or 1000 microMolar as the stock solution. How do I determine the...

11 June 2024 3,760 2 View