Why my Huh7.5 get sick and not growing after plasmid transfection with lipofectamine 3000?

01 April 2025 1 1K Report

After transfection, cells are not growing and get sick even in control empty vector transfected cell.

protocol:

1.according to the lipofectamine protocol guide

2.after 30min incubation in serum free DMEM, reaction mix was added and after 6h media was changed with complete DMEM(10% FBS, 1% Pen-strep).

Lucian Miles

Hi Sudarshan, transfection reagents all have certain cytotoxicity. Adjustments can be made from aspects such as the dosage of the reagent, the incubation time, and the cell status.

Feel free to reach out if you have other questions. Hope this helpful!

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