Hello all, I am interested in purchasing a dox-inducible system that contains either GFP or RFP to transduce into cells and use for my animal studies. The cells will also contain luciferase as I will be detecting the growth of these cells in the animals using bioluminescence imaging. Will having GFP or RFP interfere with the bioluminescence signal and alter my results?
Dear Brittany,
This literature provides a comparison between GFP luminescence and luciferase-luciferin imaging for your reference: PMID: 38925805.
GFP (Green Fluorescent Protein) has excitation/emission peaks at 397 nm and 509 nm, while RFP (Red Fluorescent Protein) exhibits peaks at 532 nm and 588 nm.
For luciferase-luciferin imaging experiments: The principle involves D-luciferin being catalyzed by luciferase to produce chemiluminescence at 560 nm. Importantly:
- No excitation light within specific wavelength ranges is required
- GFP and RFP will not generate fluorescent signals in this system、
Hope this helpful and feel free to reach out.
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