Carrot discs is the best technique especially for species with a sexual reproduction mode but you should put both male and female individuals on the discs for best results in this case.
for best results, apply auxins in an agar plate, the set the carrot, this will help to produce vegetal callus and the nematode reproduction increases
Carrot disc method is not bad to rear Pratylenchus, likely in non-contaminated environment. However, if you are in need of millions of infective Pratylenchus nematodes for advanced Seinhorst's quantitative model studies towards predicting parameter estimates for practical monitoring of economic injury levels and/or effective IPM plans against the nematode pest, Pratylenchus species on crops in the field, carrot disc methods of rearing Pratylenchus may NOT give you sufficient numbers of Pratylenchus to do these kinds of advanced quantitative nematological studies. Just simply rear them on MAIZE as it was done in the report (see the attachment). I assisted in setting up experiments for this report which was concurrently done alongside my Erasmus Mundus MSc research project, supervised by Professors Corrie H. Schomaker and Thomas H. Been, in Wageningen University in The Netherlands. It was there I realised that rearing Pratylenchus on maize produces millions of it for studies, since in field conditions Pratylenchus attacks plant roots in soils generally contaminated by several microbial organisms/pathogens.
Please, good luck,
Cheers!
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