02 February 2019 3 2K Report

Hello

I am a master student, I want to work with HEK-blue cells (cells derived from transfection of hek 293 cells, these cells are developed by invivigens). According to the supplier's recommendations the HEK-blue cells should be grown in a DMEM medium with 4.5g of glucose, in our laboratory we often work with RPMI-1640 medium, this medium contains only 2g of glucose.

I want to adapt my RPMI-1640 medium to these cells by adding glucose. is this achievable? If so how and what glucose should I add?

thank you in advance.

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