No title

21 October 2020 2,969 1 View

Optimisation of multiplex PCR on cDNA template?

I want to detect gene expression by multiplex PCR. The multiplex PCR reaction works fine on genomic DNA, but on a cDNA template the same oligo mix seems not to work as nicely, although specific...

06 November 2016 8,559 5 View

Any publication about the cost-effectiveness of multiplex real-time PCR in pathogen diagnostics?

Hello everyone! Does anyone working in the field of molecular diagnostics (particularly using qPCR and multiplex qPCR) know an updated review or comprehensive research paper with data on the...

02 March 2016 8,537 1 View

What do you mean by "interpretability" in models?

Modeling is a process of building a useful representation of a system or phenomenon. Models have a purpose, for example to precisely simulate or predict the behavior of a system. Among other...

01 July 2013 1,792 17 View

Does someone know how to separate fungal protoplasts from intact cells by centrifugation?

Our fungus is yeast-like and doesn't form spores; filtration through meshes or filters doesn't work.

21 March 2013 5,940 2 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View