There is a question surely simple
but for which I never found answers,
when a couting is realized after growth
on petri dish, how is determined the incubation
time in order to demonstrate the real rate
of microorganises present in UFC / mL.
That is, for a count of a water sample
quantifying for example 250 CFU / mL
of clostridium perfringens after incubating for
21 +/- 3H, but if I leave 26 H the count in CFU / mL
will be different and for an laboratory analysis
a product may or may not be consumable.
So how to determine the incubation time that
will directly affect the result in CFU / mL.
Thank you in advance .