There is a question surely simple

but for which I never found answers,

when a couting is realized after growth

on petri dish, how is determined the incubation

time in order to demonstrate the real rate

of microorganises present in UFC / mL.

That is, for a count of a water sample

quantifying for example 250 CFU / mL

of clostridium perfringens after incubating for

21 +/- 3H, but if I leave 26 H the count in CFU / mL

will be different and for an laboratory analysis

a product may or may not be consumable.

So how to determine the incubation time that

will directly affect the result in CFU / mL.

Thank you in advance .

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