I need to culture mammalian cells on a 60mm culture dish in an airtight container with 5% CO2 gas (5mL growth medium).
Are there any rules / published data on the ratio of medium : atmosphere that is required for optimum cell growth in closed environments? E.g. 1:20 ratio maintains stable gas concentrations for 24 hours.
Also, are there any publications describing the effects of closed culture on mammalian cells?
Thanks!
Hi, maybe this pdf can help you
https://www.idl-laborbedarf.de/H74/DWN/d19558.pdf
I have expierence with closed culture system only for delivery culture cells, I help a coworker that need to transport the lived cells. Then I used the system for some hypoxia study. maybe you can find some information about required volume of gas on hypoxia article.
Regarding study effects of closed systems I think that you can find something on web i don't understand if you need this on primary cells, for clinical purpose or something else.
For example, I can't download the article but you search something like this?
https://www.liebertpub.com/doi/abs/10.1089/hgtb.2015.080?rfr_dat=cr_pub%3Dpubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&journalCode=hgtb
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