What is the correct manner to represent the effects of a treatment in autophagic flux assay in vitro?

Dear all, In an in vitro experiment of autophagic flux assay by western blot using chloroquine, is it correct to use the LC3-II/LC3-I ratio as a manner to represent the effects of a treatment or...

07 August 2018 5,297 0 View

Does anyone know if 25 or 50 uM of chloroquine treatment could be toxic for the cells (C2C12 cells or any other cell line) in a time-course treatment?

Does anyone know if 25 or 50 uM of chloroquine treatment could be toxic for the cells (C2C12 cells or any other cell line) in a time-course treatment like 1, 3, 24, and 48 h?

05 June 2018 117 1 View

Should I add 0,1% BSA to the serum-free media to produce the C26-conditioned media or just culture the cells in the serum-free media is sufficient?

Hi everyone, I want to produce conditioned media from C26 cells for then treat C2C12 myotubes with this conditioned media. I have read some papers using serum-free media for 24h to produce C26...

04 May 2018 7,667 0 View

Is transfection efficiency of C2C12 cells with Lipofectamine 2000 or 3000 better than PEI?

Is transfection efficiency of C2C12 cells with Lipofectamine 2000 or 3000 better than PEI? And how about Fugene?

06 July 2015 8,628 7 View

How would you all interpret the following results of autophagic flux?

Dear all, How would you all interpret the following results? Please note the cells were incubated for 24 or 48h in conditioned medium in the presence of chloroquine during whole time-course of...

01 January 1970 6,310 4 View

What is the correct manner to represent the effects of a treatment in autophagic flux assay in vitro?

Dear all, In an in vitro experiment of autophagic flux assay by western blot using chloroquine, is it correct to use the LC3-II/LC3-I ratio as a manner to represent the effects of a treatment or...

01 January 1970 981 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

No title

28 February 2021 9,936 2 View