I'm trying to culture adhesion-dependent cell(satellite cell) in suspension.
I found a protocol that seeded satellite cell in petri dish for suspension culture, but I wonder if it was surface treated dish, which has charge on the surface or not.
Hello Chansik.
Most of mammalian cell culture plastics are surface-ionized, providing adherent surfaces to anchorage-dependent cells. Further enhancement of cell adherence can be obtained by coating with any proteins (i.e., gelatin, laminin, collagen, fibronectin, mainly ECM molecules, and poly-D, or L-lysine etc.).
However, the lids from culture dishes are not adherent, probably not ionized. You could use the lids.
However, culture of embryonic stem cells and other cells in suspension employ bacteriological dishes which are not adherent.
Regards.
Hi I use dishes which are not surface treated from SPL for cultivation of THP-1 pre-monocytic cell line.
polystyrene without cell surface treatment
http://www.spllifesciences.com/en/m21.php?cate=1
These plastics work well for THP-1 (not sure about satelite cells)
Hello Chansik.
Most of mammalian cell culture plastics are surface-ionized, providing adherent surfaces to anchorage-dependent cells. Further enhancement of cell adherence can be obtained by coating with any proteins (i.e., gelatin, laminin, collagen, fibronectin, mainly ECM molecules, and poly-D, or L-lysine etc.).
However, the lids from culture dishes are not adherent, probably not ionized. You could use the lids.
However, culture of embryonic stem cells and other cells in suspension employ bacteriological dishes which are not adherent.
Regards.
Corning produce ultra low adhesion tissue culture products it might be worth looking that up
Dear Chansik,
You can use ultra low attachment (ULA) plates from any company. They are standard plates for suspension culture. Though they are a little expensive. The plates are available in all well formats and also ULA T25 and T75 flasks are available with many companies.
If you have limited budget, you can coat the standard plates with PolyHema ( Poly(2-hydroxyethyl methacrylate) and that creates an ideal system for culturing cells in suspension.
Hope it helps!
Thank all of you!
Now I'm culturing satellite cell in glass bottle. but still I have to use plates to check if they are forming myosphere. and I guess surface non treated plates work well. I guess it is sort of ULA plate
Are you using sigmacote to treat your glass wear to further reduce cellular attachment??
No i didn't treat any to surface.
I made myospheres already and then transferred them to glass bottle.
- Badges
- Science topic
- Similar topics
- Biological Science
- Cell Biology
- Culture Cells