Hi! We are isolating mitochondria from platelets using nitrogen cavitation via a Parr bomb apparatus. Has anyone had experience isolating mitochondria from blood or other culture cells using this technique? Is there an optimal cell number or cell number range to be loaded? We are able to load 300 microliter aliquots of cells into our apparatus. Thank you for any assistance you may be able to provide.
Emmanuel Ifeanyi Obeagu
Hi, there has not been consensus on the cell numbers to load but most authors used 300 like you. I think you are in order but more cell optimal loading test should be done to ascertain the optimal cells to load for the best results.
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