Does who know why SDS-PAGE run like this?

samples are unaligned as picture. I guess this reason is uneven charge on machine. but i don't know precisely reason.

06 July 2018 4,137 4 View

Western blot doesn't work well, what's a problem?

Recently, I got a problem on western blot. Smaples are not apart as molecular size, and even marker is not apart from about 70kb to 130kb. I did make a new gel buffer twice, changing tris and 10%...

06 July 2018 8,629 7 View

Is intestinal epithelial cells used for reserch of colon cell profiles?

I would do mouse colon cell primary culture. So I googled mouse colon cell primary culture. But a results show me intestinal epithelial cells. these intestinal epithelial cells are colon...

03 April 2018 9,948 3 View

Any tips for mouse lung harvest?

I would extract lung from mouse. In several tries, i did extract mouse lung. But i did extract lung very poorly. So i want to extract lung fast and clearly. Would somebody give me some tips?

03 April 2018 1,900 2 View

Can I re-use a transfection supernatant?

I did transfect cell, and all cell died. And I did discard transfection supernatant, careless. Our lab has no more transfection virus, and other person has used transfection supernatant. But it...

02 March 2018 1,430 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View