How easy is this to do for someone who has never done this before? We have plenty of experience culturing cells but how easy is it to recognize when it has worked? And does anyone have a simple, detailed protocol for us to follow?
Hello
May this PDF help you
We use episomal vectors from addgene, cheap, easy and you get lots of colonies.
Thanks Saleh and Amanda-Jayne! We will have a look at these and hopefully be off and running!
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