I am trying to measure proliferation of PBMcs by flow cytometry. Does anybody have a good protocol to stimulate PBMCs with PHA and measure proliferation using the EdU Iclick kit? How long can the cells be incubated with Edu? Thank you!
Md Babu Mia
Hello Maria Nastke ,
Kindly look at the link and attached file. I hope, they will be helpful at some extent.
Regards, babu
https://www.thermofisher.com/in/en/home/references/protocols/cell-and-tissue-analysis/flow-cytometry-protocol/cell-proliferation/standard-click-it-edu-flow-cytometry-cell-proliferation-assay.html
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