I need to protocol or kit that can evaluate one gene expression on cell suspension treated with oligonucleotides in 96 well plates.
Hi Ahmed.
Here ir our lab we use Qiagen's RNA easy mini kit for RNA extraction, from bacteria and cell cultures. I could try to hel you out a bit.
What kind of genes do you want to evaluate?
João Vítor Souza · PDCD1, gene of PD-1 receptor
Hm... Let me see if I understood you correctly. Do you need a Kit that is fully ready to detect that gene or do you intend to buy especific primers, materials for RNA extraction and RT-PCR and qPCR?
I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
02 March 2021 3,060 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...
01 March 2021 9,332 6 View
28 February 2021 9,936 2 View
28 February 2021 2,534 2 View
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View