Please suggest me an inducer of PPAR alpha protein expression?

I am in a need of molecule which induces the protein expression of PPARalpha gene. Please suggest.

31 December 2018 8,215 2 View

Can someone tell me estradiol inducible gene in brain endothelial cell lines?

I want to know a gene strongly altered by 17 beta estradiol in human brain endothelial cells, so that i can take that gene as a control for the system.

04 May 2017 2,380 3 View

Could anyone guide me on procedure to dissolve and make dilutions of 17 beta estradiol?

I am trying to perform gene expression experiment with 17 beta estradiol using HepG2 Cell lines. Somehow i am not able to get any induction or akteration. It looks like i am doing something wrong...

31 December 2016 7,229 5 View

For how many days i have to culture cells in phenol red free media before giving E2 treatment?

I am using HepG2 cells as the model system

31 December 2016 9,479 1 View

How to decide time points of RNA extraction in microarray experiment?

Hello Experts, How do we decide time points of RNA extraction after giving drug to the cell lines for checking the gene expression profile in a microarray?

31 December 2015 5,992 6 View

Can I use the half life of the drug as the time point?

I don't know the time since all individual genes will be expressed at different time points. Can I use half life of the drug as the time point?

31 December 2015 6,128 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

No title

28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View