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Questions related to Column
We are doing gel filtration in our lab and encountering issues related to column length, we are not getting expected results from our process. We previously used a 24ml column with appropriate...
18 February 2022 5,107 3 View
The monomer size of my protein is 15 kDa. However, after purifying the protein using SEC, the elution volume (fraction) of the protein appears to be around 40-45 kDa compared to the SEC protein...
18 February 2022 6,474 7 View
maxquant is always incombining apl files for first search.Trying a different fasta file won't work either.The maxquant version is 2.0.3.0.Thank you all for your help!
18 February 2022 5,866 0 View
Hi all, I'm trying to separate Alpha, Beta and Gamma cyclodextrins from each other in a mixed aqueous solution. Now I know that there are slight differences in polarity of the different...
17 February 2022 3,062 1 View
I have been performing experiments in which I weigh polystyrene or polyurethane in an analytical balance, but anytime I place my sample, the weight keeps going down and does not stop. I read that...
17 February 2022 584 2 View
I have 3 columns in my dataset. Patient ID, NDC1 and NDC2. I want to find out statistical significance in cost of NDC1 and NDC2 for each patient ID. If I perform Wilcoxon Rank test, I do not get...
16 February 2022 6,610 6 View
We a re trying to separate cholesterol, cholesterolesters, triglycerides and squalene by normal-phase hplc. We only need a crude group fractionation. However, with our solvent system consisting...
15 February 2022 6,465 2 View
I have a Luna Silica column. I want to know can I use it in HILIC mode? a reason that I ask this is that column is not a HILIC column but is used in normal phase. another question is about...
14 February 2022 1,497 12 View
Hello, does anyone know how to use XRD data and CMWP (Convolutional Multiple Whole Profile) to calculate the mean square microstrain ? I am quite confused with the setting of Instrumental...
14 February 2022 9,794 2 View
I'm currently trying to use Twitter as to collect data for my research project on the use of AAVE by white gay males, but I have to admit, I find it very hard to navigate the API. Does anybody...
07 February 2022 1,875 1 View
As methanol is a highly polar solvent they generally produce polar compounds peak in gas chromatography. But we found some non-polar and volatile compounds also. For GC-MS analysis, we have used a...
05 February 2022 2,428 4 View
Is it possible to replace the Agilent HP-5MS 30m x 0.25mm x 0.25μm column with the TraceGOLD TG-5 SILMS 30m x 0.25mm x 0.25μm column in GC / MS analysis of essential oils?
01 February 2022 1,166 6 View
What can be the possible reason for this mismatch of backbone curve and hinge load path as shown in the figure. Same behavior is observed in beams as well. Because of this issue columns in my...
29 January 2022 6,158 3 View
The short column effect exist on building with different footing level (Specially when the building is resting on sloped land). Because of this the model mass participation ratio seems to be...
27 January 2022 1,621 4 View
I've already calculated the blast pressure that will act on the building. However, I am not quite sure how can I apply this load to a building. Please, help me with this one. Thanks
23 January 2022 3,800 5 View
So If a column isn't casted exactly in the intended location according to the structural drawings, and the centre of this column is moved 3 cm away from it's original location.now in the ACI code,...
20 January 2022 4,122 9 View
While trying to test out a new method (taken from the literature, nothing crazy for GCMS) The GC starts preping for the run and goes through the usual equilibration stage for pre-set amount of...
20 January 2022 822 4 View
I am running a gradient of ACN and Water in 4.6 x 250 mm C18 RP column. Column temp is 25 degrees and flow rate is 2 ml per min. I go from 50:50 (Water:ACN) to 1:99 (Water:ACN) over 10 min. I...
17 January 2022 6,911 4 View
I work with a protein that is normally well behaved, but I've recently developed the following intermittent issue: following cell lysis by sonication -- and multiple rounds of centrifugation at...
13 January 2022 1,676 4 View
Hi all, I have an issue as shown in the attached picture that occurs at the very beginning of a static non-linear column buckling analysis. I am not sure why my mesh has a local distortion there...
07 January 2022 2,949 2 View
Hi all, I was trying to impose a geometric bow imperfection to my column for non-linear buckling analysis. However, the column I am trying to analyse buckles locally (into wavy pattern) instead...
06 January 2022 5,729 6 View
Is there a nanobody directed at the 6-Histidine epitope?
05 January 2022 4,985 2 View
I am observing a high UV260 absorbance plateau during the cleaning in place of my HIC column using 1 M NaOH. This is giving me confounding results in regards to mass balance. The HIC column uses a...
04 January 2022 7,156 1 View
Dear All. I have separated 38 amino acids on HILIC column couple to QQQ followed by using 10 isotope-labeled amino acids as internal standard. However, I am not sure which formula to use to...
29 December 2021 2,496 4 View