What are the best eluent systems (solvent systems) typically used for the purification of phytochemical components (by TLC) of aqueous methanol, n-hexane, diethyl ether, chloroform, ethyl acetate, and n-butanol respectively?
The solvents you list are from a sequential or Kupchan extraction?
If so, the solvent listed would be the "weak" solvent for TLC.
Hexane -> hexane/ethyl acetate
Diethyl ether -> diethyl ether/ethyl acetate (or hexane/ethyl acetate)
Chloroform -> chloroform (or dichloromethane)/methanol
n-butanol contains a lot of water after extraction, so it is more polar than you expect. This is best run with reverse phase or HILIC
aqueous methanol will contain very polar compounds. This is another choice for reverse phase or HILIC.
The solubility of plant components in solvents is directly related to the properties of the solvent. Solvents can be divided into water, hydrophilic organic solvents, and lipophilic organic solvents. Dissolved substances also have differences in hydrophilicity and lipophilicity.
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