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Why D-Mannitol shows low antioxidant activity by DPPH assay?

I am working on DPPH assay with different antioxidants.I do not understand why some antioxidants like Ascorbic acid and Glutathione have high radical scavenging properties with IC50 less than...

08 February 2023 324 7 View

How to design the primer/probe targeting conserve sequence of a gene? How to find a conserve region of a specific gene?

We are planning to design primer/probe targeting the conserve sequence of a specific gene. We are struggling to find the conserve region. Which approach/tool is applicable to find the conserve...

07 February 2023 7,303 6 View

Is GEOCELLs will consume the loads from superstructure and transfer less loads to foundation soil ?

Dear All, As per my understanding, the Geosynthetic materials (combination of Geocell and Geogrids) will increase the bearing capacity of soils and reduce the differential settlements. I am...

07 February 2023 7,266 3 View

Proteome Discoverer. ANOVA to identify significant abundance ratio changes before or after data filtering ?

Hi, I am new to Proteome Discoverer. I have received results for phosphopeptide comparison between two mutants and control in the form of excel sheet. It shows that there are an initial 4500...

03 February 2023 8,113 0 View

Why doesn't one of the 2 pAzF UAAs label?

Dear Colleague, We are attempting to label our target with 2 separate fluorophores. However, one of the two incorporated pAzFs doesn't react with the label and we only get one-site labeling. we...

01 February 2023 5,144 0 View

What could be an alternative of Na(Hg)?

I am currently trying to make a low-valent tantalum(I) complexes. However, many reports utilized the reduction of tantalum(V) or (III) by sodium amalgam. I would avoid the use because of the...

31 January 2023 7,229 4 View

Does anyone know how to develop method of standard addition while having internal addition, GCMS?

Hi we are having Shimadzu GC 2030 and MS 8040. We are trying to develop an in Matrix method for quantification of fatty acids, by spiking the control sample (standard addition linearity). Since...

31 January 2023 8,336 5 View

Show a distinct peak while injecting HPLC Water as a sample and no peak running blank in Reverse phase HPLC using C18 column?

Wash column 100% MeOH and 100% ACN until to get baseline stable. After that equilibrate with mobile phase ( 97% 0.1% Trifluoroacetic acid (TFA)+3% ACN) for 30 minutes. After that run blank. It...

30 January 2023 8,858 3 View

What is the Specific Energy absorption of 3D printed materials?

3D printers use various polymer materials and metals. PLA, ABS, TPU, PETG, Peek, Ultem, nylon, Polyamide12, SS316, Ti-6Al-4V, Alsi10mg are some of the 3D printing materials. I need to know the...

30 January 2023 9,162 2 View

How to Inoculate a Flask containing broth?

We have a Flask that contains broth, and we want to inoculate it with Bacteria inoculum, Can we simply take a touch by the loop or by micropipette?

28 January 2023 6,938 18 View

Area integration inconsistency in GC-MS?

Hi good day everyone. I am using a Shimadzu GC-MS QP2010 SE. I am having a problem with the results of peak area. Recently, even though two duplicate samples have the same chromatogram, the...

27 January 2023 8,947 4 View

Error termination by gaussian just before the calculation ends?

I am trying to optimize a cluster based structure using DFT. However, just before the calculation

27 January 2023 396 3 View

How can I use CCA using PAST software for the analysis of different insects order with soil physical-chemical parameters????

Actually, I am using this software for the analysis of different orders of insect with seven different soil parameters and want to draw a biplot graph to use CCA in PAST, and I am not sure if I am...

24 January 2023 4,688 4 View

Preparing Carbon-dots from Ammunium citrate by using oven?

I was supposed to obtain carbon dots from Ammonium citrate dibasic as following: 1) 11.5g of Ammonium citrate dibasic + DI water 2) Stirring 3) Dilute the solution till 50ml and mixing for being...

23 January 2023 1,839 2 View

How to calculate the thickness of the sensing membrane?

I have a sensor with Ni nanoparticles on reduced graphene oxide for glycerol determination and need to calculate the thickness of the sensing membrane... Is there any way to calculate based on...

21 January 2023 1,337 4 View

Relative Antioxidant Capacity Index?

Hello everyone. I am currently working on comparing antioxidant potency between my samples. I have 4 samples = water kefir, water kefir infused with butterfly pea, water kefir infused with...

21 January 2023 5,071 5 View

While preparing biochar in a muffle furnace (without the facility of purging), the sample boxes are bursting open at around 300 deg C. Why is it so?

The biomass was pre-heated. Small steel containers of ~30g capacity were used. Thereafter, the containers were filled almost up to their brim and wrapped with Aluminium foil to further limit the...

20 January 2023 1,827 2 View

Why am I not seeing any protein band in SDS PAGE?

I am working on an extracellular bacterial protein. The exact molecular weight of the protein is unknown but it ranges from ~13 to ~185 kDa depending upon bacterial species. I was trying to...

18 January 2023 992 4 View

Which maximum (tractable) size of EBSD area is technically admitted by current techniques?

A widely spread prejudice is that EBSD is inferior to X-ray diffraction in the analysis representativeness. Meanwhile EBSD areas of millimeter sizes (with a properly increased scanning step of...

17 January 2023 1,443 10 View

How I can make concentrations for DPPH?

I'm MS student and its start of Research. Can anyone please guide me how I can make concentrations for DPPH?

17 January 2023 2,031 6 View

Is there a possibility of other functional groups (biomolecules of plant extract) present in the FTIR spectrum of metal oxide nanoparticles?

In synthesizing metal oxide nanoparticles, if the nanoparticles are calcinated at 400 oC. Then, is there a possibility of other functional groups (biomolecules of plant extract) in the FTIR...

15 January 2023 417 5 View

Can i Quantify a Known Nitrosamine (NDSRI-API Related) without Reference standard ?

In our product NDSRI (API related nitrosamine) is present. To develop a method to quantify that Nitrosamine i dont have a standard in my hand (Not even available in the market). How can i approch...

12 January 2023 8,454 7 View

How HLB and CMC values effect the adsoption of Tween 20 molecule on hydrophobic molecule?

I would like to consult you about some technical issues regarding Tween 20 usage. We are using Sigma-Tween 20 EMPROVE ESSENTIAL for dissolution studies of our product. The product is a form of...

10 January 2023 4,533 2 View

Q-Exactive calibration error for Mass resolution Dependency too high, can anyone help?

Hi, After performing my annual cleaning on the QE and calibrated, it as passes the positive cal but when performing the basic (negative) Analyzer accuracy it has failed for Mass resolution...

09 January 2023 2,200 1 View

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