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Questions related to Column
I have five different columns from Dr.Maish: 1) ReproSil - XR 120 NH2 2) Reprosil 80 NH2 3) Reprospher 100 CN 4) Reprosil - Pur 120 NH2 5) Reprosil Saphir 100 NH2 4) Reprosil - Pur 120 NH2 Can I...
09 June 2022 2,461 0 View
While identifying the Differentially expressed genes (DEGs), I have found in the literature for setting the parameters of logFC >1 and p-value
09 June 2022 2,999 0 View
I'm preparing a Eugenol nanoemulsion using Tween 20 as the surfactant. Ethanol is employed as cosurfactant. I wanted to know if Tween 20 acts as an antimicrobial agent and can contribute to the...
07 June 2022 5,860 1 View
My protein forms a loose debris after dialysis, which reduces its performance. What can be done to prevent this?
04 June 2022 6,977 1 View
Hi, any idea why Maxquant (Version see above) is not reporting iBAQ values although I specified iBAQ in the parameters? Thanks in advance!
19 May 2022 5,638 5 View
I want to prepare a standard solution of tyrosine for measuring protease activity and do a calibration curve but I don't know which is the best concentration to prepare and which step should I follow?
03 May 2022 6,969 5 View
I obtained an EIS data when I am doing research on biopolymers for corrosion inhibition. But I can not find a good fit of equivalent circuit. Could any body help me with it? I have attached the...
26 April 2022 8,105 3 View
If yes, what concentration did you use that and how did you get rid of the AHA for enzyme activity? I tried using 5uM of AHA, got a good yield, proceeded to removing the AHA with gravity desalting...
20 April 2022 6,417 0 View
I want to ask a question, about the loading protocol, which is very important, mostly in literature, 2 types of instrumental assemblie were used, 1) first one is to apply the axial load on the...
17 April 2022 5,406 2 View
When trying to analyze bullet impact with LS Dyna it is passing through the plate without destruction. I am trying to model a bullet impact in LS-DYNA using CONTACT_AUTOMATIC_SURFACE_TO_SURFACE....
30 March 2022 7,843 4 View
Hello Mr. Varaprasad, In your paper "Analysis of cough and analgesic range of pharmaceutical active ingredients using RP-HPLC method" you mentioned that solution A is a buffer, is it ammonium...
21 March 2022 5,759 2 View
Hello, There is lacI protein which is binding to the expressed recombinant protein after purification using Protino Ni-TED 2000 packed columns. I have tried gel filtration to separate the proteins...
20 March 2022 2,649 5 View
Greetings, we ran gel electrophoresis in the lab on pig red cells ghosts, the first column is the maker. The second and fourth columns are of concern. The second column contains ghost cells, the...
16 March 2022 6,231 2 View
I tried both FAM-aptamer and Texas red-aptamer (1 ul of 100uM, dilute with 200 ul PBS). When I add 500ng protein (5 ul) to the solution, I found the fluorescence from dye was about 15% decreased....
16 March 2022 3,878 1 View
I have a large list of factor-names in an excel sheet, and there are similar and dissimilar factors across different samples. The similar/dissimilar factors are in different rows. I would like to...
13 March 2022 3,200 3 View
I would like to run LC-MS/MS with a 300um column, but the commercially available ones are only 15 cm. There are many papers that discuss packing 50-150um columns in 365um OD capillary, but has...
11 March 2022 2,439 1 View
Hi. i want to purify a 75kDa protein with a poly His Taq. can you help me find the best method?
10 March 2022 1,518 3 View
I have histones extracted with acid precipitation from hek cells. What are the most appropriate spin columns for purification? I will not perform protein digestion. Thanks
09 March 2022 1,437 1 View
I have a bacterial metabolite. I want to identify both polar and non-polar metabolites present in the sample. Do I need to separate metabolites into polar and non-polar categories run separately...
07 March 2022 7,079 5 View
I am quite new on this subject. Suppose the expected failure of the joint is "beam flexural failure," do I need to use the hysteretic curve constructed from column drift or beam displacement?
27 February 2022 2,311 0 View
I am looking to remove sodium bicarbonate from my reaction mixture. I reacted galactosamine hydrochloride with acetic anhydride under basic conditions provided by sodium bicarbonate in water. Is...
26 February 2022 5,387 3 View
How to condition a new C18 column? What are the prerequisites and cautions? What are the solvent concentrations you suggest?
22 February 2022 3,621 3 View
Hi, I am trying to cast Tris-Acetate Gel because I am working with (very) high molecular weight proteins. However, I am not finding any literature on the recipe of the Stacking gel necessary for...
22 February 2022 3,870 1 View
Hello Has anyone used a Carboxen PLOT 1010 column to separate gases such as carbon monoxide, methane and carbon dioxide on GC FID. If so what is the lowest concentration you have managed to get...
20 February 2022 4,070 0 View