How can we optimize the activity of a cell type-specific promoter driving a GFP reporter? We observed a remarkably weak expression of the reporter gene in the cell line. In contrast, native animal cells exhibit significantly higher promoter activity.
Robert Adolf Brinzer
This would indicate that you are missing some upstream or downstream enhancers in your promoter sequence. How many kbp did you clone? Sometimes including the first/second intron can help.
An alternative strategy is to use your promoter for specificity which drives T7 polymerase with an NLS that can then obtain strong expression of GFP under a T7 promoter.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Column
More Yan Zhang's questions See All
Similar questions and discussions