Contact experts in Column to get answers
3,165 views 3,233 posts
Questions related to Column
Hello I want to apply Biogel P-6 as a SEC resin. But I don't know how do its CIP. Last time, I used ethanol20% for CIP and its flowrate has decreased significantly to 0.5 ml/min due to very high...
30 September 2023 6,103 0 View
If we are trying to quantify hydrogen produced per electrode area in a cell or stack, what is the good way of doing it?
27 September 2023 3,472 2 View
I'm using Mirdeep2 to determine the transcript abundances of Mirna, however I've been working with single-end data and only getting counts from the forward strand. Are there any options in...
26 September 2023 7,229 0 View
While lysing the leaf sample for RNA isolation a jelly-like substance was released which led to a low yield of RNA. How to overcome this issue. PS: RNA isolation was carried out using nucleospin...
22 September 2023 8,454 3 View
To get better digestibility of the plant proteins (Fava bean protein isolates). What heat treatment should I give before the IVD (In vitro digestion) process ?
21 September 2023 7,215 0 View
Hi, I am doing DNA conjugation onto 15nm gold nanoparticles using the freezing method. However, after overnight incubation gold nanoparticles turn blue (even without adding any salt). I wonder if...
20 September 2023 2,881 4 View
I am interested in utilizing a screw-press to effectively dewater a slurry. Allow me to provide you with the specifications of the slurry in question: - Flow: The slurry has a flow rate of 50...
20 September 2023 5,132 4 View
hello, i am intersted in the cause of getting higher concentration of RNA when using the acid phenol chlorofom extraction to the qiagen kit.. i am extracing RNA from cells and i tried both method...
19 September 2023 6,600 0 View
The cell membrane is made up of phospholipid, which contain net negative charges. So, what will be the overall charge on the surface of cell surface.
13 September 2023 9,467 1 View
THANK FULL TO YOU
12 September 2023 1,969 2 View
Hello everyone, I'm currently establishing a new project using the human ovarian cancer cell line HEY. Unfortunately it turned out that they are negative for both of the two surface markers...
12 September 2023 6,421 2 View
Thank you
12 September 2023 8,063 1 View
Dear Researchgate-Community, I plan to start writing my master's thesis soon in the field of business communication. As I want to use corpus linguistics on Elon Musks tweets in this context, I...
09 September 2023 8,417 0 View
I collected approx. 15 adult Culiseta mosquitoes from a forest and I am trying to obtain egg rafts after blood feeding the mosquitoes. They feed readily on the blood that is offered to them, but I...
06 September 2023 1,629 0 View
STEP 1: Evaluate the quality of the PROM development, using COSMIN box 1 is just used for us to understand how to give score to the PROM development study? In table1, two columns named "content...
05 September 2023 7,622 0 View
how to use a material studio to build a Montmorillonite ( bentonite ) model? with any exchangeable ion, like Na+, K+, Fe3+ etc. and thank u
04 September 2023 2,591 1 View
I'm currently finalising the formatting for an MRes thesis. Now, I may have experience with statistical analysis and detailed report writing, but this relatively small issue has stumped me,...
01 September 2023 5,250 3 View
Hello. I have to check the packed cell volume of a bacterial suspension, but I do not have access to a Hopkins tube. Is there ay way to convert OD600 to packed cell volume? I know this might be...
26 August 2023 9,128 0 View
Do greenhouse gases affect climate change and how does ozone layer depletion contribute to global warming?
23 August 2023 7,428 18 View
Dear all, regarding instrument modification, is there any rule of thumb on how far we could do the modification in an instrument/ scale? Thank you in advance for your respond.
21 August 2023 8,075 0 View
Dear All (those working in the area of modelling of gasifiers), I have been unsuccessful in securing the article authored by Babu et al titled, 'Fluidization correlations for coal gasification...
21 August 2023 1,894 0 View
there is scenario, so i have an experiment that accumulates cadmium in plants with different amendments and they turn out to be different sizes will the amount of cd differ in each 1 gram of plant...
19 August 2023 3,850 3 View
Need some guide how to simulate the stone column model of manuscript "Simplified Plane-Strain Modeling of Stone-Column Reinforced Ground - DOI: 10.1061/(ASCE)1090-0241(2008)134:2(185)" with PLAXIS...
19 August 2023 5,424 1 View
NA
17 August 2023 3,125 0 View