Hi Berkay Alpay, it is not uncommon to observe variations in the molecular weight of protein bands detected by different antibodies, even when targeting the same protein. These variations can arise due to various factors such as antibody specificity, post-translational modifications, or isoform-specific differences. In the case of GAT-1 (SLC6A1), different antibodies may recognize different isoforms or post-translationally modified forms of the protein, resulting in variations in the detected molecular weight. Additionally, differences in sample preparation and experimental conditions can also influence the observed band migration.

To address this issue and reconcile the differences in molecular weight, you can consider the following steps:

• Verify antibody specificity: Ensure that the antibody you are using (antiSLC6A1 from Elabscience) has been validated for GAT-1 detection in previous studies or references. Reviewing literature or consulting with experts in the field can provide insights into the reliability of the antibody.
• Positive control: Include a positive control in your experiments, such as a cell lysate or tissue known to express GAT-1 at the expected molecular weight. This will help confirm the antibody's specificity and aid in determining the appropriate molecular weight of the GAT-1 band.
• Compare with other studies: Look for published research that has used similar experimental conditions and the same or different GAT-1 antibodies. Compare their results to gain a broader understanding of the molecular weight variations observed in different studies.
• Consider alternative detection methods: If the variations in molecular weight persist, you may explore alternative techniques like mass spectrometry or other forms of protein identification to confirm the identity and molecular weight of the detected band.