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Questions related to Column
Hi, I have been having problems for the last month in the resolutiono of the peaks of the first three windows, and they start to come out well after minute 6. I have changed column, septum,...
09 January 2023 2,215 4 View
Dear all, I am having a technical problem with our FACS Canto II. It seemed somehow the device picked up some air bubbles and now it does not complete the fluidics start up neither the purge. I...
08 January 2023 4,433 4 View
I am interested in Thermo Scientific EASY-Spray 150umx15 cm LC Columns. I want to use the capillary column with the nanoLC system. Using the capillary column with the nanoLC system, is it possible...
06 January 2023 9,176 4 View
Soil and rock anchors have pull-out specifications that depend on many variables, such as the type of anchor mechanism, looseness of soils, depth of compaction, etc. However there must be some...
06 January 2023 4,984 3 View
Hello, I have been attempting to purify a recombinant protein fused at the C-terminus with Twin-StrepTag. For that I am using the Strep-Tactin XT 4Flow High Capacity Spin Column Kit by IBA...
05 January 2023 2,013 7 View
I have seen several publications where both are used, but I would like a selection criteria, please.
04 January 2023 9,981 4 View
Hi everyone I want to determination 16 PAHs in edible oil by GC-MS. But for this reason that matrix is so difficult, I can't have a good recovery and sensitivity. Please introduce methods for...
03 January 2023 6,493 4 View
Hi everyone, Currently we have a UV fluctation problem on AKTAPrime Plus. This fluctation is only happening with the SEC Columns (Superose 6 Increase 3.2/300 and Superose 6 Increase 10/300 GL)....
02 January 2023 7,874 0 View
The maximum strength in elastic region for alloy
30 December 2022 7,446 3 View
am trying to optimize the geometry of (C5H14N2)[CuCl4] using g09. However, the calculation is not converging at any level of theory in 250 cycles. I am new to this field and I think I have made a...
29 December 2022 1,255 4 View
How does heavy metal affect water quality and can heavy metal pollution be prevented in water and can heavy metal poisoning is prevented?
26 December 2022 2,828 4 View
I performed BApNA assay for soybean samples. I also took the readings using spectrophotometer. But how should I calculate the TUI for the same samples? For BLANK, I took 300ul of trypsin + 2.5 ml...
25 December 2022 2,476 2 View
hello everyone, I made refolding buffer with cystine and cysteine and cystine was solved successfully in the buffer. today after 12h I took out my refolding buffer and added protein from +4, I saw...
21 December 2022 406 3 View
An industrial town produces 18,000 cubic meters of wastewater per day. The contamination load of COD is 8300 mg/liter And the amount of BOD contamination is 3,900 mg/liter. The amount of nitrogen...
20 December 2022 5,412 16 View
Most of the codes in the examples in the literature are presented for discrete variables. While the main challenge in solving Bayesian networks is the use of continuous variables. Marco's chain...
19 December 2022 6,448 4 View
Hey there every researchers Can I give up the step 4 of this protocol of RQ1 RNase-Free DNase (Promegt) for my RNA extraction samples? Because I had experiences in Leb that 65 degree temperature...
18 December 2022 2,665 4 View
Is it possible that adsorption capacity (qe) decreases with increase in adsorbent dosage at fixed adsorbate conc, while the removal efficiency increases?
15 December 2022 1,982 6 View
I am evaluating the digestibility of egg yolk product by INFOGEST 2. I used a blank with enzymes and SSf /SGF and SFI. I measured the nitrogen content during digestion with DUMAS. then, from each...
14 December 2022 686 1 View
Goodmorning, I work with SEC-FPLC (column: Superose 6 Increase 10/300 GL) and I used it to run two different samples: 1) Cetuximab (145,78 kDa) and, as you can see from the image attached it...
13 December 2022 1,228 0 View
I need to make 100mL of the following recipe: 20mM Tris-HCl, pH 7.5 100mM NaCl 6mM MgCl2. How many grams of NaCl and MgCl2, do I need? I'm very rusty on undergrad chemistry! Thank you!!!
12 December 2022 4,965 0 View
Hey jelly community! I was wondering whether you could help me ID the species depicted in the attached photos?! The specimens have been collected in October and November this year in the Bay of...
11 December 2022 3,935 3 View
I want to apply PEG/Dextran system to separate specific-sized molecules from a bio-complex system containing various biomolecular components. I would like to know how Dextran concentration and...
09 December 2022 5,089 0 View
In My Lab, we use HPLC made by SHIMADZU. so books or manuals related to this company can help.
07 December 2022 2,087 2 View
I have applied both pseudo first and pseudo second order linear models on my data. Calculated and experimental qe values of pseudo first order are very near to each other. However, in pseudo...
07 December 2022 8,504 6 View