For lc50 study on fish against bacteria, the test performed referring to OD reading at 625nm. Is it acceptable method?

I would like to clarify regarding a method used to perform LC50 to infect fish by a particular bacteria strain. I prepared an overnight bacteria culture, collect the pellet from overnight culture...

05 December 2020 7,220 1 View

How deep learning with IoT can be applied in agriculture?suggest any specific application of agriculture ?

Application of agriculture

01 January 2019 9,107 3 View

Can any one suggest me alternate substrate for alpha amylase (PPA) assay?

20 March 2017 5,761 3 View

What is the reason for peak splitting in XRD? Can reitveld refinement be done for the same?

18 November 2016 8,730 16 View

Can we consider negative result of sample in antioxidant assay?

In all antioxidant assay for calculation below mentioned formula is used % of Inhibition = CONTROL O.D - Sample O.D/ CONTROL O.D X 100 But, In this case I got negative result of sample because...

10 August 2015 9,438 15 View

Which control should be used for my calculation: a positive which has standard compound or a negative control which has reagents only

19 May 2015 3,845 2 View

How do you plot a tafel plot?

12 January 2015 1,498 6 View

How to synthesize graphene from graphite?

I want to synthesise graphene from graphite. Can anyone give me an easy procedure for that?

26 February 2014 1,914 10 View

Plasmid DNA isolation for shRNA vectors giving very low yield with Stbl4 transformation

My vector in which I cloned shRNA primers have a very high copy. I cloned shRNA plasmids and then tried to transform them in Stbl4 but I'm getting very very low or almost negligible concentration...

07 April 2013 3,819 15 View

Whats the best way to transfect Hela Cells? We are using PEI but efficiency is very low.

We are using Hela cells for checking localization of proteins by Immunofluorescence and transfecting these cells with PEI, but efficiency is just 30 %. How to improve it? Should we use another...

24 September 2012 2,606 16 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

No title

25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View