Hello Dr. Niladri Sekhar Das,

You may use RNAlater solution. While RNAlater is primarily designed for preserving RNA, it can also be used to preserve DNA. The solution effectively inactivates nucleases, which are enzymes that degrade both RNA and DNA, thus protecting both types of nucleic acids from degradation. It can preserve samples at room temperature for several weeks. It can be used as a transport medium to collect and transport tissue samples from remote locations or when immediate processing is not possible, and therefore, makes it convenient for field studies. 

Samples can be directly submerged in RNAlater at the time of collection. While it is effective for shorter periods at room temperature, RNAlater allows for long-term storage of samples at -20°C or -80°C. 

Regards,

Malcolm Nobre

Hello Dr.Niladri Sekhar Das,

Though I can't make sure this way can have a great effect, I suggest you first frozen your sample with liquid nitrogen and then transport it in solid carbon dioxide.

Take 250ul cells from eppendrof into cryovials

700ul FBS +50ul DMSO

parafilm the cryovials ..this whole process called cryopresrervation …further we used for dna and rna extraction.

Yes, there are a number of commercially available transport media specifically designed to preserve tissue samples for DNA and RNA extraction. These media help preserve the integrity of nucleic acids by inhibiting enzymatic degradation processes (by RNases and DNases) and stabilizing cells or tissues after collection. Here are some common examples of suitable transport media or preservation solutions:

1. RNAprotect Tissue Reagent (Qiagen)

Use: Specifically designed for RNA preservation.

How it works: This reagent immediately stabilizes RNA in fresh tissue samples while inactivating RNases.

Benefits:

Compatible with standard RNA extraction kits.

Allows storage at room temperature for several days before processing.

Limitations: Less suitable for simultaneous DNA extraction.

2. Allprotect Tissue Reagent (Qiagen)

Use: Simultaneous preservation of DNA, RNA and proteins in the same sample.

How it works: This reagent preserves nucleic acids and proteins by inhibiting degrading enzymes.

Benefits:

Versatile for various molecular analyses.

Stabilizes samples at room temperature for up to several weeks.

Limitations: More expensive than specialized solutions for DNA or RNA only.